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. 1975 Sep;72(9):3416–3420. doi: 10.1073/pnas.72.9.3416

In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

J R Cameron, S M Panasenko, I R Lehman, R W Davis
PMCID: PMC433004  PMID: 1103146

Abstract

DNA from lambdagt-lambdaB bacteriophage was cleaved with EcoRI endonuclease and fragments from EcoRI-digested E. coli DNA were inserted. This DNA was used to infect E. coli, and phages containing the gene for DNA ligase were isolated by genetic selection. Two different hybrids were found with the same E. coli segment inserted in opposite orientations. Both hybrids produced similar levels of ligase as measured in crude extracts of infected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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