Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Sep;72(9):3541–3545. doi: 10.1073/pnas.72.9.3541

Cell surface species-specific high affinity receptors for discoidin: developmental regulation in Dictyostelium discoideum.

R W Reitherman, S D Rosen, W A Frasier, S H Barondes
PMCID: PMC433031  PMID: 1059141

Abstract

Vegetative (noncohesive) D. discoideum cells and cells at several stages during the development of cohesiveness were fixed with glutaraldehyde, and their agglutinability by purified carbohydrate-binding proteins (lectins) from slime molds and plants was determined. The two purified lectins from D. discoideum, called discoidin I and II, were poor agglutinins of fixed vegetative D. discoideum cells and potent agglutinins of fixed cohesive D. discoideum cells. The increased agglutinability of D. discoideum cells by discoidin I or discoidin II with differentiation paralleled discoidin appearance in cell extracts and development of cohesiveness. The lectin of another slime mold, Polysphondylium pallidum (pallidin) and the lectin of Ricinus communis (RCA-I) were also more potent agglutinins of fixed differentiated D. discoideum cells than of fixed vegetative D. discoideum cells. However, wheat germ agglutinin was much more potent with fixed vegetative cells. The association constant (Ka) of discoidin I and discoidin II for fixed differentiated D. discoideum cells was in the range of 10(9) M-1, more than twenty-fold higher than the Ka of these lectins for fixed vegetative cells. The Ka of discoidin I and discoidin II for fixed differentiated P. pallidum cells was an order of magnitude lower than for fixed differentiated D. discoideum cells. The Ka of pallidin for fixed differentiated P. pallidum cells was about 4 X 10(9) M-1 and was an order of magnitude lower for fixed differentiated D. discoideum cells. Since previous studies showed that the lectin of each of these slime mold species is detectable on the surface of cohesive cells, the observation of species-specific receptors on the surface of cohesive cells suggests a basis for species-specific cohesion.

Full text

PDF
3541

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BONNER J. T., ADAMS M. S. Cell mixtures of different species and strains of cellular slime moulds. J Embryol Exp Morphol. 1958 Jun;6(2):346–356. [PubMed] [Google Scholar]
  2. GERISCH G. [Cell functions and change in cell function in the development of Dictyostelium discoideum. V. Stagespecific cell contact formation and its quantitative evaluation]. Exp Cell Res. 1961 Dec;25:535–554. doi: 10.1016/0014-4827(61)90189-6. [DOI] [PubMed] [Google Scholar]
  3. Kornfeld S., Rogers J., Gregory W. The nature of the cell surface receptor site for Lens culinaris phytohemagglutinin. J Biol Chem. 1971 Nov;246(21):6581–6586. [PubMed] [Google Scholar]
  4. Nicolson G. L. The interactions of lectins with animal cell surfaces. Int Rev Cytol. 1974;39:89–190. doi: 10.1016/s0074-7696(08)60939-0. [DOI] [PubMed] [Google Scholar]
  5. Reitherman R. W., Rosen S. D., Barondes S. H. Lectin purification using formalinised erythrocytes as a general affinity adsorbant. Nature. 1974 Apr 12;248(449):599–600. doi: 10.1038/248599a0. [DOI] [PubMed] [Google Scholar]
  6. Rosen S. D., Kafka J. A., Simpson D. L., Barondes S. H. Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2554–2557. doi: 10.1073/pnas.70.9.2554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rosen S. D., Simpson D. L., Rose J. E., Barondes S. H. Carbohydrate-binding protein from Polysphondylium pallidum implicated in intercellular adhesion. Nature. 1974 Nov 8;252(5479):128, 149-50. doi: 10.1038/252128a0. [DOI] [PubMed] [Google Scholar]
  8. STECK T. L., HOELZLWALLACH D. F. THE BINDING OF KIDNEY-BEAN PHYTOHEMAGGLUTININ BY EHRLICH ASCITES CARCINOMA. Biochim Biophys Acta. 1965 Mar 8;97:510–522. [PubMed] [Google Scholar]
  9. Simpson D. L., Rosen S. D., Barondes S. H. Discoidin, a developmentally regulated carbohydrate-binding protein from Dictyostelium discoideum. Purification and characterization. Biochemistry. 1974 Aug 13;13(17):3487–3493. doi: 10.1021/bi00714a011. [DOI] [PubMed] [Google Scholar]
  10. Weeks G. Agglutination of growing and differentiating cells of Dictyostelium discoideum by concanavalin A. Exp Cell Res. 1973 Feb;76(2):467–470. doi: 10.1016/0014-4827(73)90406-0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES