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. 1975 Oct;72(10):3934–3938. doi: 10.1073/pnas.72.10.3934

Translation of hormone-induced messenger RNA in amphibian oocytes: I. Induction by estrogen of messenger RNA encoded for vitellogenic protein in the liver of the male African clawed toad (Xenopus laevis).

K D Lanclos, T H Hamilton
PMCID: PMC433111  PMID: 1060075

Abstract

Induction of the synthesis of the vitellogenic proteins, lipovitellin and phosvitin, in the liver of the male African clawed toad (Xenopus laevis) was investigated as a function of time after treatment with estradiol-17beta [1,3,5(10)-estratriene-3,17beta-diol]. The appearance of mRNAs encoded for lipovitellin and phosvitin in the cytoplasmic fraction of the liver was assayed by microinjections of hepatic mRNA preparation [either polyribosomes or poly(A)-rich RNA] into oocytes obtained from mature female toads. Oocytes were then incubated in the presence of radioactive amino acid(s) at 19 degrees for periods of time varying from 4 to 18 hr after microinjection. The results show that at 2 hr after hormone treatment more mRNA was present in the cytoplasm, and that from 2 to 72 hr after treatment the level of induced mRNA increased almost linearly to 110% above the control values. Experiments employing specific lipovitellin antiserum indicated no radioactive lipovitellin among the proteins synthesized in oocytes microinjected with hepatic mRNAs isolated from 3 to 9 hr after hormone treatment. However, a marked synthesis of immunoprecipitable, radioactive lipovitellin and an enhanced incorporation of [3H]serine occurred in the oocytes microinjected with hepatic mRNA preparations obtained from toads treated with hormone for 12 or more hr. The identities of the proteins encoded by the mRNAs induced early in estrogen action (2-9 hr) in the male amphibian liver are unknown. It is surmised that some of these proteins may function in the regulation of the subsequent synthesis of the vitellogenic proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blobel G., Potter V. R. Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo. J Mol Biol. 1967 Sep 28;28(3):539–542. doi: 10.1016/s0022-2836(67)80103-7. [DOI] [PubMed] [Google Scholar]
  2. Brawerman G., Mendecki J., Lee S. Y. A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry. 1972 Feb 15;11(4):637–641. doi: 10.1021/bi00754a027. [DOI] [PubMed] [Google Scholar]
  3. Cohen M. E., Hamilton T. H. Sequential stimulation of synthesis of two specific cytoplasmic proteins in early estrogen action. Biochem Biophys Res Commun. 1975 May 19;64(2):633–639. doi: 10.1016/0006-291x(75)90368-x. [DOI] [PubMed] [Google Scholar]
  4. Follett B. K., Nicholls T. J., Redshaw M. R. The vitellogenic response in the South African clawed toad (Xenopus laevis Daudin). J Cell Physiol. 1968 Oct;72(2 Suppl):91+–91+. doi: 10.1002/jcp.1040720408. [DOI] [PubMed] [Google Scholar]
  5. Gurdon J. B. Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes. J Embryol Exp Morphol. 1968 Nov;20(3):401–414. [PubMed] [Google Scholar]
  6. Gurdon J. B., Lane C. D., Woodland H. R., Marbaix G. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature. 1971 Sep 17;233(5316):177–182. doi: 10.1038/233177a0. [DOI] [PubMed] [Google Scholar]
  7. Katzenellenbogen B. S., Gorski J. Estrogen action in vitro. Induction of the synthesis of a specific uterine protein. J Biol Chem. 1972 Feb 25;247(4):1299–1305. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lane C. D., Marbaix G., Gurdon J. B. Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes. J Mol Biol. 1971 Oct 14;61(1):73–91. doi: 10.1016/0022-2836(71)90207-5. [DOI] [PubMed] [Google Scholar]
  10. Moar V. A., Gurdon J. B., Lane C. D., Marbaix G. Translational capacity of living frog eggs and oocytes, as judged by messenger RNA injection. J Mol Biol. 1971 Oct 14;61(1):93–103. doi: 10.1016/0022-2836(71)90208-7. [DOI] [PubMed] [Google Scholar]
  11. O'Malley B. W., Means A. R. Female steroid hormones and target cell nuclei. Science. 1974 Feb 15;183(4125):610–620. doi: 10.1126/science.183.4125.610. [DOI] [PubMed] [Google Scholar]
  12. STAEHELIN T., WETTSTEIN F. O., OURA H., NOLL H. DETERMINATION OF THE CODING RATIO BASED ON MOLECULAR WEIGHT OF MESSENGER RIBONUCLEIC ACID ASSOCIATED WITH ERGOSOMES OF DIFFERENT AGGREGATE SIZE. Nature. 1964 Jan 18;201:264–270. doi: 10.1038/201264a0. [DOI] [PubMed] [Google Scholar]
  13. STEELE W. J., OKAMURA N., BUSCH H. EFFECTS OF THIOACETAMIDE ON THE COMPOSITION AND BIOSYNTHESIS OF NUCLEOLAR AND NUCLEAR RIBONUCLEIC ACID IN RAT LIVER. J Biol Chem. 1965 Apr;240:1742–1749. [PubMed] [Google Scholar]
  14. Schutz G., Beato M., Feigelson P. Isolation of eukaryotic messenger RNA on cellulose and its translation in vitro. Biochem Biophys Res Commun. 1972 Nov 1;49(3):680–689. doi: 10.1016/0006-291x(72)90465-2. [DOI] [PubMed] [Google Scholar]
  15. WALLACE R. A. STUDIES ON AMPHIBIAN YOLK. IV. AN ANALYSIS OF THE MAIN-BODY COMPONENT OF YOLK PLATELETS. Biochim Biophys Acta. 1963 Aug 13;74:505–518. doi: 10.1016/0006-3002(63)91393-3. [DOI] [PubMed] [Google Scholar]
  16. Wallace R. A. Resolution and isolation of avian and amphibian yolk-granule proteins using TEAE-cellulose. Anal Biochem. 1965 May;11(2):297–311. doi: 10.1016/0003-2697(65)90018-7. [DOI] [PubMed] [Google Scholar]
  17. Wittliff J. L., Kenney F. T. Regulation of yolk protein synthesis in amphibian liver. I. Induction of lipovitellin synthesis by estrogen. Biochim Biophys Acta. 1972 May 29;269(3):485–492. doi: 10.1016/0005-2787(72)90136-0. [DOI] [PubMed] [Google Scholar]
  18. Wittliff J. L., Lee K. L., Kenney F. T. Regulation of yolk protein synthesis in amphibian liver. II. Elevation of ribonucleic acid synthesis by estrogen. Biochim Biophys Acta. 1972 May 29;269(3):493–504. doi: 10.1016/0005-2787(72)90137-2. [DOI] [PubMed] [Google Scholar]
  19. Wittliff J. L., Zelson P. R. Estrogen specificity of the induction of lipovitellin syntheses and evidence for a specific estrogen-binding component. Endocr Res Commun. 1974;1(2):117–131. doi: 10.3109/07435807409053821. [DOI] [PubMed] [Google Scholar]

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