Abstract
A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse cells; (2) its antiviral activity was species specific; (3) its activity was completely neutralized by mouse anti-interferon serum. The synthesis of interferon in this cell-free system requires the presence of spermine.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bassin R. H., Phillips L. A., Kramer M. J., Haapala D. K., Peebles P. T., Nomura S., Fischinger P. J. Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1520–1524. doi: 10.1073/pnas.68.7.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Both G. W., Banerjee A. K., Shatkin A. J. Methylation-dependent translation of viral messenger RNAs in vitro. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189–1193. doi: 10.1073/pnas.72.3.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies J. W., Kaesberg P. Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ. J Gen Virol. 1974 Oct;25(1):11–20. doi: 10.1099/0022-1317-25-1-11. [DOI] [PubMed] [Google Scholar]
- Davies J. W., Kaesberg P. Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo. J Virol. 1973 Dec;12(6):1434–1441. doi: 10.1128/jvi.12.6.1434-1441.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gozes I., Schmitt H., Littauer U. Z. Translation in vitro of rat brain messenger RNA coding for tubulin and actin. Proc Natl Acad Sci U S A. 1975 Feb;72(2):701–705. doi: 10.1073/pnas.72.2.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knight E., Jr Heterogeneity of purified mouse interferons. J Biol Chem. 1975 Jun 10;250(11):4139–4144. [PubMed] [Google Scholar]
- Marcu K., Dudock B. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974 Nov;1(11):1385–1397. doi: 10.1093/nar/1.11.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montagnier L., Collandre H., De Maeyer-Guignard J., De Maeyer E. Two forms of mouse interferon messenger RNA. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1031–1038. doi: 10.1016/s0006-291x(74)80083-5. [DOI] [PubMed] [Google Scholar]
- Prives C. L., Aviv H., Paterson B. M., Roberts B. E., Rozenblatt S., Revel M., Winocour E. Cell-free translation of messenger RNA of simian virus 40: synthesis of the major capsid protein. Proc Natl Acad Sci U S A. 1974 Feb;71(2):302–306. doi: 10.1073/pnas.71.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts B. E., Gorecki M., Mulligan R. C., Danna K. J., Rozenblatt S., Rich A. Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A. 1975 May;72(5):1922–1926. doi: 10.1073/pnas.72.5.1922. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts B. E. Tobacco mosaic virus RNA directs the synthesis of a coat protein peptide in a cell-free system from wheat. J Mol Biol. 1973 Nov 15;80(4):733–742. doi: 10.1016/0022-2836(73)90206-4. [DOI] [PubMed] [Google Scholar]
- Shih D. S., Kaesberg P. Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799–1803. doi: 10.1073/pnas.70.6.1799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sipe J. D., de Maeyer-Guignard J., Fauconnier B., de Maeyer E. Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1037–1040. doi: 10.1073/pnas.70.4.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wagner R. R., Huang A. S. Reversible inhibition of interferon synthesis by puromycin: evidence for an interferon-specific messenger RNA. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1112–1118. doi: 10.1073/pnas.54.4.1112. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Maeyer-Guignard J., de Maeyer E., Montagnier L. Interferon messenger RNA: translation in heterologous cells. Proc Natl Acad Sci U S A. 1972 May;69(5):1203–1207. doi: 10.1073/pnas.69.5.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]