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. 1975 Oct;72(10):4028–4032. doi: 10.1073/pnas.72.10.4028

Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells.

J P Merlie, A Sobel, J P Changeux, F Gros
PMCID: PMC433131  PMID: 1060086

Abstract

Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of the purified radioactive species is established by cosedimentation of the [35S]receptor with [3H]alpha-toxin binding activity on sucrose gradients and by crossreaction with antiserum to purified acetylcholine receptor of Electrophorus electricus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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