Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Oct;72(10):4123–4127. doi: 10.1073/pnas.72.10.4123

Purification and synthesis of eosinophilotactic tetrapeptides of human lung tissue: identification as eosinophil chemotactic factor of anaphylaxis.

E J Goetzl, K F Austen
PMCID: PMC433151  PMID: 1060093

Abstract

Preferential eosinophil chemotactic activity exhibiting a molecular weight comparable to that released from sensitized human lung fragments challenged with specific antigen and designated eosinophil chemotactic factor of anaphylaxis has been isolated from extracts of human lung fragments by sequential purification on Sephadex G-25, Dowex-1, Sephadex G-10, and paper chromatography. Two eosinophilotactic tetrapeptides of amino acid sequence Val-Gly-Ser-Glu and Ala-Gly-Ser-Glu were recovered from the extracts in 4-12% overall yield of the low molecular weight peak from Sephadex G-25. Purified eosinophil chemotactic factor of anaphylaxis and the synthetic tetrapeptides were maximally active in amounts from 0.1 to 1.0 nmol per chemotactic chamber, and the activity was dependent on both the NH2-terminal and the COOH-terminal residues. Both natural and synthetic peptides were preferentially chemotactic for eosinophils and rendered them unresponsive to a subsequent stimulus.

Full text

PDF
4123

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYDEN S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962 Mar 1;115:453–466. doi: 10.1084/jem.115.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Day R. P. Eosinophil cell separation from human peripheral blood. Immunology. 1970 Jun;18(6):955–959. [PMC free article] [PubMed] [Google Scholar]
  3. Goetzl E. J., Austen K. F. A neutrophil-immobilizing factor derived from human leukocytes. I. Generation and partial characterization. J Exp Med. 1972 Dec 1;136(6):1564–1580. doi: 10.1084/jem.136.6.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gutte B., Merrifield R. B. The synthesis of ribonuclease A. J Biol Chem. 1971 Mar 25;246(6):1922–1941. [PubMed] [Google Scholar]
  5. HIRS C. H. The oxidation of ribonuclease with performic acid. J Biol Chem. 1956 Apr;219(2):611–621. [PubMed] [Google Scholar]
  6. Hartley B. S. Strategy and tactics in protein chemistry. Biochem J. 1970 Oct;119(5):805–822. doi: 10.1042/bj1190805f. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaliner M., Wasserman S. I., Austen K. F. Immunologic release of chemical mediators from human nasal polyps. N Engl J Med. 1973 Aug 9;289(6):277–281. doi: 10.1056/NEJM197308092890601. [DOI] [PubMed] [Google Scholar]
  8. Kay A. B., Austen K. F. The IgE-mediated release of an eosinophil leukocyte chemotactic factor from human lung. J Immunol. 1971 Sep;107(3):899–902. [PubMed] [Google Scholar]
  9. Kay A. B., Stechschulte D. J., Austen K. F. An eosinophil leukocyte chemotactic factor of anaphylaxis. J Exp Med. 1971 Mar 1;133(3):602–619. doi: 10.1084/jem.133.3.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lewis R. A., Goetzl E. J., Wasserman S. I., Valone F. H., Rubin R. H., Austen K. F. The release of four mediators of immediate hypersensitivity from human leukemic basophils. J Immunol. 1975 Jan;114(1 Pt 1):87–92. [PubMed] [Google Scholar]
  11. Matsuo H., Fujimoto Y., Tatsuno T. A novel method for the determination of C-terminal amino acid in polypeptides by selective tritium labelling. Biochem Biophys Res Commun. 1966 Jan 4;22(1):69–74. doi: 10.1016/0006-291x(66)90604-8. [DOI] [PubMed] [Google Scholar]
  12. ORTEN A. U., DOPPKE H. J., SPURRIER H. H. INCREASED SENSITIVITY IN AUTOMATIC AMINO ACID ANALYSIS OBTAINED WITH A SPECIAL HYBRID RECORDER. Anal Chem. 1965 Apr;37:623–624. doi: 10.1021/ac60223a061. [DOI] [PubMed] [Google Scholar]
  13. Orange R. P., Austen W. G., Austen K. F. Immunological release of histamine and slow-reacting substance of anaphylaxis from human lung. I. Modulation by agents influencing cellular levels of cyclic 3',5'-adenosine monophosphate. J Exp Med. 1971 Sep 1;134(3 Pt 2):136s–148s. [PubMed] [Google Scholar]
  14. Schiffmann E., Corcoran B. A., Wahl S. M. N-formylmethionyl peptides as chemoattractants for leucocytes. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1059–1062. doi: 10.1073/pnas.72.3.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schiffmann E., Showell H. V., Corcoran B. A., Ward P. A., Smith E., Becker E. L. The isolation and partial characterization of neutrophil chemotactic factors from Escherichia coli. J Immunol. 1975 Jun;114(6):1831–1837. [PubMed] [Google Scholar]
  16. Wasserman S. I., Goetzl E. J., Austen K. F. Inactivation of slow reacting substance of anaphylaxis by human eosinophil arylsulfatase. J Immunol. 1975 Feb;114(2 Pt 1):645–649. [PubMed] [Google Scholar]
  17. Wasserman S. I., Goetzl E. J., Austen K. F. Preformed eosinophil chemotactic factor of anaphylaxis (ECF-A). J Immunol. 1974 Jan;112(1):351–358. [PubMed] [Google Scholar]
  18. Wasserman S. I., Goetzl E. J., Ellman L., Austen K. F. Tumor-associated eosinophilotactic factor. N Engl J Med. 1974 Feb 21;290(8):420–424. doi: 10.1056/NEJM197402212900802. [DOI] [PubMed] [Google Scholar]
  19. Wasserman S. I., Goetzl E. J., Kaliner M., Austen K. F. Modulation of the immunological release of the eosinophil chemotactic factor of anaphylaxis from human lung. Immunology. 1974 Apr;26(4):677–684. [PMC free article] [PubMed] [Google Scholar]
  20. Wasserman S. I., Whitmer D., Geotzl E. J., Austen K. F. Chemotactic deactivation of human eosinophils by the eosinophil chemotactic factor of anaphylaxis (38527). Proc Soc Exp Biol Med. 1975 Jan;148(1):301–306. doi: 10.3181/00379727-148-38527. [DOI] [PubMed] [Google Scholar]
  21. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES