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. 1973 Jan;70(1):18–21. doi: 10.1073/pnas.70.1.18

Translational Control of Specific Uterine Protein Synthesis After Estrogen Induction

Anthony B DeAngelo 1,*, George I Fujimoto 1,†
PMCID: PMC433174  PMID: 4509650

Abstract

The rate of leucine incorporation into a specific estrogen-induced protein of immature rat isolated by gel electrophoresis declines rapidly between 2-4 hr after estrogen stimulation in vivo followed by incubation in vitro. Actinomycin D present during the in vitro phase prevents this decline, and elicits a “superinduction” effect at 2 hr. Labeled induced protein remains stable during this time period, indicating that its decline is due to a reduction in synthesis capacity for the inducible protein, rather than to its degradation. A second injection of hormone at 3 hr has no effect on the reduced level of synthesis capacity for induced protein noted at 4 hr in the rat uterus.

Keywords: rat, gel electrophoresis, actinomycin D, 17β-estradiol

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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