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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Jan;70(1):66–70. doi: 10.1073/pnas.70.1.66

Electron Spin Resonance Studies of Spin-Labeled Mammalian Cells by Detection of Surface-Membrane Signals

Joseph Kaplan 1,*, Peter G Canonico 1, William J Caspary 1,†
PMCID: PMC433185  PMID: 4346040

Abstract

Lipid-soluble spin labels were incorporated into human lymphocytes and mouse L-cells and the resulting electron spin resonance spectra were compared with spectra obtained from similarly labeled human erythrocytes. Spin labels were found in all subcellular fractions of the nucleated cells that contained membranes. Spinlabeled cells remained viable and capable of replicating in vitro. Electron spin resonance signals from spin-labeled nucleated cells underwent a time- and temperature-dependent decay that was reversed by bathing the cells in K3Fe(CN)6. The demonstration of a relative cell impermeability to ferricyanide, as measured by both colorimetric and radioisotopic label methods, indicated that only spin-labeled molecules in the surface membrane were reactivated when ferricyanide was added to spin-labeled cells after the electron spin resonance signal had decayed.

Keywords: lipid-soluble labels, living nucleated cells, erythrocytes, potassium ferricyanide

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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