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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Jan;70(1):84–87. doi: 10.1073/pnas.70.1.84

Genetic Transformation in Escherichia coli K12

Sharon D Cosloy 1, Michio Oishi 1
PMCID: PMC433189  PMID: 4630612

Abstract

An auxotrophic strain of E. coli K12 treated with CaCl2 was transformed for several markers at a frequency of up to 10-6 per recipient cell by a DNA preparation isolated from a prototrophic strain. The transforming activity of the DNA preparation was eliminated by treatment with DNase, heat, or sonication, whereas RNase or Pronase treatment had little effect. Two closely linked genetic markers (leu and ara) showed a high degree of cotransformation linkage when high molecular weight DNA was used, but the linkage was almost completely eliminated when sheared, smaller molecular weight DNA was used. There is genetic evidence that the transformation is a result of the replacement of the preexisting genetic marker on the chromosome by that of the donor DNA.

Keywords: DNA, CaCl2, recombination, genetic linkage

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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