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. 1973 Jan;70(1):103–107. doi: 10.1073/pnas.70.1.103

Inhibition of a DNA Polymerase from Bacillus subtilis by Hydroxyphenylazopyrimidines

Kenneth B Gass 1,2, Robert L Low 1,2, Nicholas R Cozzarelli 1,2
PMCID: PMC433193  PMID: 4630611

Abstract

The antimicrobial agent, 6-(p-hydroxyphenylazo)-uracil, specifically inhibits DNA polymerase III of Bacillus subtilis with a Ki of less than 1 μM. The inhibition requires prior reduction of the drug, is reversible, and is competitive with dGTP. High amounts of dATP prevent inhibition by the closely related drug, 6-(p-hydroxyphenylazo)-isocytosine. A model is presented in which the inhibitors base-pair with the template while part of a ternary complex with the enzyme. These results imply that DNA polymerase III of B. subtilis is necessary for chromosomal replication.

Keywords: base-pairing, antimicrobials, DNA replication

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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