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. 1973 Jan;70(1):129–133. doi: 10.1073/pnas.70.1.129

Ultraviolet-Stimulated DNA Synthesis in Toluenized Escherichia coli Deficient in DNA Polymerase I

Warren E Masker 1, Philip C Hanawalt 1
PMCID: PMC433199  PMID: 4567330

Abstract

Ultraviolet (UV)-stimulated nonconservative DNA synthesis has been observed in E. coli rendered permeable to nucleoside triphosphates by exposure to toluene. This synthesis is detected in toluenized cells in which the background of UV-independent DNA synthesis is reduced by use of dnaB mutants temperature-sensitive for DNA replication and additionally deficient in DNA polymerase I. UV-stimulated nonconservative synthesis is also seen in polA dnaE mutants that are deficient in two of the three known DNA polymerases. The observed UV-stimulated repair-like synthesis requires the presence of the four deoxyribonucleoside triphosphates and ATP. This mode of synthesis appears to differ from the ATP-independent nonconservative DNA synthesis previously reported to occur in toluenized bacteria.

Keywords: temperature-sensitive mutants, nonconservative, dnaE, repair synthesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonhoeffer F. DNA transfer and DNA synthesis during bacterial conjugation. Z Vererbungsl. 1966;98(2):141–149. doi: 10.1007/BF00897186. [DOI] [PubMed] [Google Scholar]
  2. Burger R. M. Toluene-treated Escherichia coli replicate only that DNA which was about to be replicated in vivo. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2124–2126. doi: 10.1073/pnas.68.9.2124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHAMBERLIN M., BALDWIN R. L., BERG P. AN ENZYMICALLY SYNTHESIZED RNA OF ALTERNATING BASE SEQUENCE: PHYSICAL AND CHEMICAL CHARACTERIZATION. J Mol Biol. 1963 Oct;7:334–349. doi: 10.1016/s0022-2836(63)80028-5. [DOI] [PubMed] [Google Scholar]
  4. Campbell J. L., Soll L., Richardson C. C. Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090–2094. doi: 10.1073/pnas.69.8.2090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper P. K., Hanawalt P. C. Heterogeneity of patch size in repair replicated DNA in Escherichia coli. J Mol Biol. 1972 Jun 14;67(1):1–10. doi: 10.1016/0022-2836(72)90381-6. [DOI] [PubMed] [Google Scholar]
  6. Cooper P. K., Hanawalt P. C. Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli. Proc Natl Acad Sci U S A. 1972 May;69(5):1156–1160. doi: 10.1073/pnas.69.5.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Couch J., Hanawalt P. C. DNA repair replication in temperature-sensitive DNA synthesis deficient bacteria. Biochem Biophys Res Commun. 1967 Dec 29;29(6):779–784. doi: 10.1016/0006-291x(67)90287-2. [DOI] [PubMed] [Google Scholar]
  8. Gefter M. L., Hirota Y., Kornberg T., Wechsler J. A., Barnoux C. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150–3153. doi: 10.1073/pnas.68.12.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Howard-Flanders P. DNA repair. Annu Rev Biochem. 1968;37:175–200. doi: 10.1146/annurev.bi.37.070168.001135. [DOI] [PubMed] [Google Scholar]
  10. Kato T., Kondo S. Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis. J Bacteriol. 1970 Nov;104(2):871–881. doi: 10.1128/jb.104.2.871-881.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kohiyama M., Cousin D., Ryter A., Jacob F. Mutants thermosensibles d'Escherichia coli K 12. I. Isolement et caractérisation rapide. Ann Inst Pasteur (Paris) 1966 Apr;110(4):465–486. [PubMed] [Google Scholar]
  12. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  13. Matsushita T., White K. P., Sueoka N. Chromosom replication in toluenized Bacillus subtilis cells. Nat New Biol. 1971 Jul 28;232(30):111–114. doi: 10.1038/newbio232111a0. [DOI] [PubMed] [Google Scholar]
  14. Mordoh J., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli. V. Incorporation of deoxynucleoside triphosphates by DNA thermosensitive mutants of Escherichia coli also lacking DNA polymerase activity. Proc Natl Acad Sci U S A. 1970 Oct;67(2):773–778. doi: 10.1073/pnas.67.2.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Moses R. E., Richardson C. C. Replication and repair of DNA in cells of Escherichia coli treated with toluene. Proc Natl Acad Sci U S A. 1970 Oct;67(2):674–681. doi: 10.1073/pnas.67.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Noguti T., Kada T. Semi-in vitro repair of radiation-induced damage in transforming DNA of Bacillus subtilis. J Mol Biol. 1972 Jun 28;67(3):507–512. doi: 10.1016/0022-2836(72)90467-6. [DOI] [PubMed] [Google Scholar]
  17. Oishi M. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292–1299. doi: 10.1073/pnas.64.4.1292. [DOI] [PMC free article] [PubMed] [Google Scholar]

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