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. 1973 Jan;70(1):215–219. doi: 10.1073/pnas.70.1.215

Self-Complementarity of Terminal Sequences Within Plus or Minus Strands of Adenovirus-Associated Virus DNA*

Frank J Koczot 1, Barrie J Carter 1, Claude F Garon 1, James A Rose 1
PMCID: PMC433218  PMID: 4509654

Abstract

At least 70% of plus or minus strands of adenovirus-associated virus DNA contain self-complementary sequences at or near their termini. Self-annealing of these sequences generates circular molecules that are closed by duplex, hydrogen-bonded segments. The self-annealed segments are sensitive to exonuclease III and have a thermal stability comparable to that of double-stranded DNA molecules. Length measurements of double-stranded adenovirus-associated virus DNA molecules show a bimodal distribution, with the larger component being 10% shorter than SV40 DNA.

The presence of self-complementary terminal sequences in single-stranded molecules of viral DNA has been observed previously only with DNA from adenoviruses. It is thus especially notable that adenovirus-associated virus replication is unconditionally dependent on a helper adenovirus. A possible role for terminal self-complementary sequences in viral DNA replication is suggested.

Keywords: circular DNA, electron microscopy, length measurements, exonuclease III

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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