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. 1973 Jan;70(1):220–224. doi: 10.1073/pnas.70.1.220

Qualitative Differences in the N-Acetyl-D-galactosaminyltransferases Produced by Human A1 and A2 Genes

H Schachter *, M A Michaels *, Christine A Tilley †, Marie C Crookston ‡, J H Crookston ‡
PMCID: PMC433219  PMID: 4509655

Abstract

This study describes the kinetic properties of N-acetyl-D-galactosaminyltransferase in serum from subjects with blood groups A1 and A2. When the A1 and A2 enzymes were compared, with lacto-N-fucopentaose I and 2′-fucosyllactose as acceptors, the enzymes differed in their cation requirements, pH optima, and Km values. The two acceptors competed for the same transferase. Mixing experiments showed that the lower activity of the A2 enzyme could not be attributed to a modifier or inhibitor in serum. It was concluded that the A1 and A2 enzymes differ qualitatively.

Keywords: blood group A, serum glycosyltransferases, glycoprotein biosynthesis, milk oligosaccharides

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Selected References

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