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. 1973 Feb;70(2):315–319. doi: 10.1073/pnas.70.2.315

Translation of Bacteriophage Qβ RNA by Cytoplasmic Extracts of Mammalian Cells

Trudy G Morrison 1, Harvey F Lodish 1
PMCID: PMC433247  PMID: 4568725

Abstract

Cytoplasmic extracts from Krebs II mouse ascites cells and from L cells translate messenger RNA from coliphage Qβ with fidelity to produce products that migrate on polyacrylamide gels with those products directed by Qβ RNA in an Escherichia coli cell-free system. The mammalian cell extracts correctly initiate and terminate Qβ coat protein synthesis, as shown by: (i) [3H]lysine-and [3H]arginine-labeled tryptic peptides derived from the coat-sized product resemble these from authentic Qβ coat protein, (ii) Qβ coat (which contains methionine only at the N-terminal end) can be radioactively labeled with methionine only if the methionine is formylated, and (iii) L cell extracts directed by Qβ am-11 (an amber mutant in the coat protein) RNA make no completed coat-sized material, but do make a peptide the size of the authentic amber coat fragment.

Keywords: L cells, Krebs ascites cells, coat protein, tryptic peptides, N-formylmethionine

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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