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. 1973 Feb;70(2):343–346. doi: 10.1073/pnas.70.2.343

Control of Normal Differentiation of Myeloid Leukemic Cells to Macrophages and Granulocytes

Eitan Fibach 1, Makoto Hayashi 1, Leo Sachs 1
PMCID: PMC433254  PMID: 4510279

Abstract

Cells from a myeloid leukemic line in culture can be induced by the differentiation-inducing protein MGI to form colonies with normal differentiation to mature macrophages and granulocytes. This line consisted of clones that can be induced to undergo normal cell differentiation (D+ clones) and clones (D- clones) that were not inducible. D+ clones were able to undergo differentiation to both macrophages and granulocytes. Normal differentiation was induced even in clones that were no longer diploid. D+ clones can segregate some D- progeny, and D- clones can segregate some D+ progeny. This, therefore, provides a system for studies on the genetic and chemical control of cell differentiation in leukemic cells.

Keywords: mice, chromosome number, differentiation-inducing protein

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caspersson T., Zech L., Johansson C. Differential binding of alkylating fluorochromes in human chromosomes. Exp Cell Res. 1970 Jun;60(3):315–319. doi: 10.1016/0014-4827(70)90523-9. [DOI] [PubMed] [Google Scholar]
  2. Fibach E., Landau T., Sachs L. Normal differentiation of myeloid leukaemic cells induced by a differentiation-inducing protein. Nat New Biol. 1972 Jun 28;237(78):276–278. doi: 10.1038/newbio237276a0. [DOI] [PubMed] [Google Scholar]
  3. GINSBURG H., SACHS L. FORMATION OF PURE SUSPENSIONS OF MAST CELLS IN TISSUE CULTURE BY DIFFERENTIATION OF LYMPHOID CELLS FROM THE MOUSE THYMUS. J Natl Cancer Inst. 1963 Jul;31:1–39. [PubMed] [Google Scholar]
  4. Ginsburg H., Sachs L. Destruction of mouse and rat embryo cells in tissue culture by lymph node cells from unsensitized rats. J Cell Physiol. 1965 Oct;66(2):199–219. doi: 10.1002/jcp.1030660207. [DOI] [PubMed] [Google Scholar]
  5. Hitotsumachi S., Rabinowitz Z., Sachs L. Chromosomal control of chemical carcinogenesis. Int J Cancer. 1972 Mar 15;9(2):305–315. doi: 10.1002/ijc.2910090208. [DOI] [PubMed] [Google Scholar]
  6. Hitotsumachi S., Rabinowitz Z., Sachs L. Ciromosomal control of reversion in transformed cells. Nature. 1971 Jun 25;231(5304):511–514. doi: 10.1038/231511a0. [DOI] [PubMed] [Google Scholar]
  7. Huebner R. J., Todaro G. J. Oncogenes of RNA tumor viruses as determinants of cancer. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1087–1094. doi: 10.1073/pnas.64.3.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ichikawa Y. Differentiation of a cell line of myeloid leukemia. J Cell Physiol. 1969 Dec;74(3):223–234. doi: 10.1002/jcp.1040740303. [DOI] [PubMed] [Google Scholar]
  9. Ichikawa Y., Pluznik D. H., Sachs L. In vitro control of the development of macrophage and granulocyte colonies. Proc Natl Acad Sci U S A. 1966 Aug;56(2):488–495. doi: 10.1073/pnas.56.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Landau T., Sachs L. Activation of a differentiation-inducing protein by adenine and adenine containing nucleotides. FEBS Lett. 1971 Oct 1;17(2):339–341. doi: 10.1016/0014-5793(71)80182-5. [DOI] [PubMed] [Google Scholar]
  11. Landau T., Sachs L. Characterization of the inducer required for the development of macrophage and granulocyte colonies. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2540–2544. doi: 10.1073/pnas.68.10.2540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Metcalf D., Moore M. A., Warner N. L. Colony formation in vitro by myelomonocytic leukemic cells. J Natl Cancer Inst. 1969 Oct;43(4):983–1001. [PubMed] [Google Scholar]
  13. Paran M., Sachs L., Barak Y., Resnitzky P. In vitro induction of granulocyte differentiation in hematopoietic cells from leukemic and non-leukemic patients. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1542–1549. doi: 10.1073/pnas.67.3.1542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Paran M., Sachs L. The continued requirement for inducer for the development of macrophage and granulocyte colonies. J Cell Physiol. 1968 Dec;72(3):247–250. doi: 10.1002/jcp.1040720312. [DOI] [PubMed] [Google Scholar]
  15. Pluznik D. H., Sachs L. The cloning of normal "mast" cells in tissue culture. J Cell Physiol. 1965 Dec;66(3):319–324. doi: 10.1002/jcp.1030660309. [DOI] [PubMed] [Google Scholar]
  16. Pluznik D. H., Sachs L. The induction of clones of normal mast cells by a substance from conditioned medium. Exp Cell Res. 1966 Oct;43(3):553–563. doi: 10.1016/0014-4827(66)90026-7. [DOI] [PubMed] [Google Scholar]
  17. Rabinowitz Z., Sachs L. Control of the reversion of properties in transformed cells. Nature. 1970 Jan 10;225(5228):136–139. doi: 10.1038/225136a0. [DOI] [PubMed] [Google Scholar]
  18. Sumner A. T., Evans H. J., Buckland R. A. New technique for distinguishing between human chromosomes. Nat New Biol. 1971 Jul 7;232(27):31–32. doi: 10.1038/newbio232031a0. [DOI] [PubMed] [Google Scholar]
  19. Todaro G. J., Huebner R. J. N.A.S. symposium: new evidence as the basis for increased efforts in cancer research. Proc Natl Acad Sci U S A. 1972 Apr;69(4):1009–1015. doi: 10.1073/pnas.69.4.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]

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