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. 1973 Feb;70(2):421–424. doi: 10.1073/pnas.70.2.421

Regulation of the Expression of the N Gene of Bacteriophage Lambda

Jack Greenblatt 1,*
PMCID: PMC433273  PMID: 4568728

Abstract

A quantitative assay for the N protein of bacteriophage λ has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free protein-synthesizing system from Escherichia coli programmed with λN- DNA the λ endolysin is made only if N protein is added to the reaction.

The rate of synthesis of N protein in vivo is negatively controlled by the products of the CI and tof genes of the phage. Furthermore, N protein activity is extremely unstable in vivo. During normal cell growth at 35°, the half-life of N protein is about 2 min.

Keywords: N protein assay, in vitro endolysin synthesis, tof gene control, functionally unstable protein

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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