Abstract
The contraction of isolated smooth-muscle cells has been correlated with evagination of the cell membrane, a marked change in myofilament orientation, and a decrease in cellular volume. Both localized and full contractions have been elicited in the same cell by varying the intensity of electrical stimulation. These results may be explained by a model of the smooth-muscle cell in which the contractile apparatus extends between attachment sites on the cell membrane that are relatively closely spaced.
Keywords: cell volume, cell membrane, myofilaments, contractile mechanism
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Selected References
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- BLINKS J. R. INFLUENCE OF OSMOTIC STRENGTH ON CROSS-SECTION AND VOLUME OF ISOLATED SINGLE MUSCLE FIBRES. J Physiol. 1965 Mar;177:42–57. doi: 10.1113/jphysiol.1965.sp007574. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bagby R. M., Young A. M., Dotson R. S., Fisher B. A., McKinnon K. Contraction of single smooth muscle cells from Bufo marinus stomach. Nature. 1971 Dec 10;234(5328):351–352. doi: 10.1038/234351a0. [DOI] [PubMed] [Google Scholar]
- Cooke P. H., Fay F. S. Thick myofilaments in contracted and relaxed mammalian smooth muscle cells. Exp Cell Res. 1972;71(2):265–272. doi: 10.1016/0014-4827(72)90292-3. [DOI] [PubMed] [Google Scholar]
- Gordon A. M., Huxley A. F., Julian F. J. The variation in isometric tension with sarcomere length in vertebrate muscle fibres. J Physiol. 1966 May;184(1):170–192. doi: 10.1113/jphysiol.1966.sp007909. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelly R. E., Rice R. V. Ultrastructural studies on the contractile mechanism of smooth muscle. J Cell Biol. 1969 Sep;42(3):683–694. doi: 10.1083/jcb.42.3.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane B. P. Alterations in the cytologic detail of intestinal smooth muscle cells in various stages of contraction. J Cell Biol. 1965 Oct;27(1):199–213. doi: 10.1083/jcb.27.1.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PEASE D. C., MOLINARI S. Electron microscopy of muscular arteries; pial vessels of43 the cat and monkey. J Ultrastruct Res. 1960 Jun;3:447–468. doi: 10.1016/s0022-5320(60)90022-8. [DOI] [PubMed] [Google Scholar]
- ROSENBLUTH J. SMOOTH MUSCLE: AN ULTRASTRUCTURAL BASIS FOR THE DYNAMIC OF ITS CONTRACTION. Science. 1965 Jun 4;148(3675):1337–1339. doi: 10.1126/science.148.3675.1337. [DOI] [PubMed] [Google Scholar]
- Small J. V., Squire J. M. Structural basis of contraction in vertebrate smooth muscle. J Mol Biol. 1972 Jun 14;67(1):117–149. doi: 10.1016/0022-2836(72)90390-7. [DOI] [PubMed] [Google Scholar]