Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Mar;70(3):714–717. doi: 10.1073/pnas.70.3.714

Initiation and Continuation of DNA Replication Are Not Associated with the Nuclear Envelope in Mammalian Cells

Gary E Wise 1,*, D M Prescott 1
PMCID: PMC433342  PMID: 4268475

Abstract

For determination of whether DNA replication is initiated at the nuclear envelope, synchronized Chinese hamster ovary cells labeled with [3H]thymidine were examined by electron microscope radioautography. The cells were synchronized initially by mitotic shake-off and held at the G1-S border by 5-fluorodeoxyuridine plus amethopterin. Cells were fixed at 1, 5, 10, and 30 min after the inhibitors were counteracted with [3H]thymidine. Radioautographic silver grains in each case were present over the more central parts of nuclei and were generally absent from the region of the nuclear envelope. We conclude that neither initiation nor continuation of DNA replication is associated with the nuclear envelope.

Keywords: cell synchrony, CHO cells, G1-S border, electron microscope radioautography

Full text

PDF
714

Images in this article

715Image
on p.715
715Image
on p.715
716Image
on p.716
716Image
on p.716

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bostock C. J., Prescott D. M., Kirkpatrick J. B. An evaluation of the double thymidine block for synchronizing mammalian cells at the G1-S border. Exp Cell Res. 1971 Sep;68(1):163–168. doi: 10.1016/0014-4827(71)90599-4. [DOI] [PubMed] [Google Scholar]
  2. Comings D. E., Kakefuda T. Initiation of deoxyribonucleic acid replication at the nuclear membrane in human cells. J Mol Biol. 1968 Apr 14;33(1):225–229. doi: 10.1016/0022-2836(68)90290-8. [DOI] [PubMed] [Google Scholar]
  3. Erlandson R. A., de Harven E. The ultrastructure of synchronized HeLa cells. J Cell Sci. 1971 Mar;8(2):353–397. doi: 10.1242/jcs.8.2.353. [DOI] [PubMed] [Google Scholar]
  4. Fakan S., Turner G. N., Pagano J. S., Hancock R. Sites of replication of chromosomal DNA in a eukaryotic cell. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2300–2305. doi: 10.1073/pnas.69.8.2300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Flickinger C. J. The postnatal development of the Sertoli cells of the mouse. Z Zellforsch Mikrosk Anat. 1967;78(1):92–113. doi: 10.1007/BF00344405. [DOI] [PubMed] [Google Scholar]
  6. Huberman J. A., Tsai A., Deich R. A. DNA replication sites within nuclei of mammalian cells. Nature. 1973 Jan 5;241(5384):32–36. doi: 10.1038/241032a0. [DOI] [PubMed] [Google Scholar]
  7. Jacob F., Ryter A., Cuzin F. On the association between DNA and membrane in bacteria. Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):267–278. doi: 10.1098/rspb.1966.0029. [DOI] [PubMed] [Google Scholar]
  8. Klein A., Bonhoeffer F. DNA replication. Annu Rev Biochem. 1972;41(10):301–332. doi: 10.1146/annurev.bi.41.070172.001505. [DOI] [PubMed] [Google Scholar]
  9. Ockey C. H. Distribution of DNA replicator sites in mammalian nuclei. II. Effects of prolonged inhibition of DNA synthesis. Exp Cell Res. 1972 Jan;70(1):203–213. doi: 10.1016/0014-4827(72)90198-x. [DOI] [PubMed] [Google Scholar]
  10. Tobey R. A., Anderson E. C., Petersen D. F. Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells. J Cell Physiol. 1967 Aug;70(1):63–68. doi: 10.1002/jcp.1040700109. [DOI] [PubMed] [Google Scholar]
  11. Williams C. A., Ockey C. H. Distribution of DNA replicator sites in mammalian nuclei after different methods of cell synchronization. Exp Cell Res. 1970 Dec;63(2):365–372. doi: 10.1016/0014-4827(70)90224-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES