Abstract
Reversible light-induced absorbance changes were observed in the phototactic organism Dictyostelium discoideum. Irradiation of cells with light in the 520- to 600-nm region results in an absorbance increase at 411 nm, which decays in darkness with a half-time of about 7 sec. A similar light-induced absorbance change was observed in a 12,000 × g pellet of a cell-free homogenate and in a soluble fraction obtained after sonication of the 12,000 × g pellet. Dithionite had to be added to the cell-free extracts in order for the light-induced change to decay in darkness. The absorbance change was maximally elicited by light in the region of 560 nm. A similar spectral sensitivity was found for the phototactic migration of the pseudoplasmodia. The agreement between the spectral sensitivities of the two processes suggests that the absorbance change is associated with phototaxis.
Keywords: photoreceptor, pseudoplasmodia, slime mold
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Selected References
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