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. 1973 Mar;70(3):890–893. doi: 10.1073/pnas.70.3.890

A Stopped-Flow Apparatus with Light-Scattering Detection and Its Application to Biochemical Reactions

Detlev Riesner 1, Hans Buenemann 1
PMCID: PMC433382  PMID: 4577138

Abstract

A stopped-flow apparatus utilizing light-scattering for following the progress of a reaction is described. The method is applicable to all reactions that result in a significant change of the average molecular weight. It was possible due to several modifications of a conventional stopped-flow system to obtain a sensitivity comparable to that of commercial instruments for static light-scattering measurements. Experiments on three reactions are reported: association and dissociation of mercury ligands with DNA, dissociation of the dimers of DNA-dependent RNA polymerase, and complex formation of tRNASer (yeast) with the cognate aminoacyl-tRNA synthetase. The changes in the intensities of the scattered light are calculated and compared with the measured amplitudes.

Keywords: laser excitation, filtration, biopolymers, amplitudes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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