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. 1973 Mar;70(3):919–923. doi: 10.1073/pnas.70.3.919

Population Analysis of the Deinduction Kinetics of Galactose Long-Term Adaptation Mutants of Yeast

Shinji Tsuyumu 1, Bruce G Adams 1
PMCID: PMC433388  PMID: 4577139

Abstract

By use of a selective galactose agar medium containing ethidium bromide, a population analysis of the deinduction kinetics of yeast galactose long-term adaptation mutants (gal 3) has been done. It was first determined that the gal 3 mutation is specific to the yeast galactose system and that induced cultures of gal 3 strains are capable of growth on galactose agar medium containing ethidium bromide, whereas noninduced cultures are not. Population analyses of induced gal 3 strains under going deinduction in the absence of galactose demonstrate that a minimum number of five induction units per cell are required for induction of the galactose system. It is concluded that: these induction units are actively synthesized only in the presence of inducer and are diluted out through cell division; they are stable under nongrowing conditions; they are heterogeneous in nature; at most two of the five minimum units are products of the gal 2 locus; and the other units may be three of one type, one of one type and two of another, or one each of three different types.

Keywords: ethidium bromide, heterogeneous induction units, endogenous inducer

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams B. G. Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects. J Bacteriol. 1972 Aug;111(2):308–315. doi: 10.1128/jb.111.2.308-315.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHN M., HORIBATA K. Analysis of the differentiation and of the heterogeneity within a population of Escherichia coli undergoing induced beta-galactosidase synthesis. J Bacteriol. 1959 Nov;78:613–623. doi: 10.1128/jb.78.5.613-623.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DOUGLAS H. C., CONDIE F. The genetic control of galactose utilization in Saccharomyces. J Bacteriol. 1954 Dec;68(6):662–670. doi: 10.1128/jb.68.6.662-670.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldring E. S., Grossman L. I., Marmur J. Petite mutation in yeast. II. Isolation of mutants containing mitochondrial deoxyribonucleic acid of reduced size. J Bacteriol. 1971 Jul;107(1):377–381. doi: 10.1128/jb.107.1.377-381.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JOHNSTON J. R., MORTIMER R. K. Use of snail digestive juice in isolation of yeast spore tetrads. J Bacteriol. 1959 Aug;78:292–292. doi: 10.1128/jb.78.2.292-292.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Novick A., Weiner M. ENZYME INDUCTION AS AN ALL-OR-NONE PHENOMENON. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):553–566. doi: 10.1073/pnas.43.7.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SPIEGELMAN S., SUSSMAN R. R., PINSKA E. On the cytoplasmic nature of "long-term adaptation" in yeast. Proc Natl Acad Sci U S A. 1950 Nov;36(11):591–606. doi: 10.1073/pnas.36.11.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SPIEGELMAN S. The particulate transmission of enzyme-forming capacity in yeast. Cold Spring Harb Symp Quant Biol. 1951;16:87–98. doi: 10.1101/sqb.1951.016.01.008. [DOI] [PubMed] [Google Scholar]
  9. Spiegelman S., Delorenzo W. F., Campbell A. M. A Single-Cell Analysis of the Transmission of Enzyme-Forming Capacity in Yeast. Proc Natl Acad Sci U S A. 1951 Aug;37(8):513–524. doi: 10.1073/pnas.37.8.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Spiegelman S., Delorenzo W. F. Substrate Stabilization of Enzyme-Forming Capacity During the Segregation of a Heterozygote. Proc Natl Acad Sci U S A. 1952 Jul;38(7):583–592. doi: 10.1073/pnas.38.7.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]
  12. Wu H. C., Kalckar H. M. Endogenous induction of the galactose operon in Escherichia coli K12. Proc Natl Acad Sci U S A. 1966 Mar;55(3):622–629. doi: 10.1073/pnas.55.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]

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