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. 1973 Mar;70(3):941–943. doi: 10.1073/pnas.70.3.941

Flavin 1, N6-Ethenoadenine Dinucleotide: Dynamic and Static Quenching of Fluorescence

Jorge R Barrio 1, Glen L Tolman 1, Nelson J Leonard 1,*, Richard D Spencer 1, Gregorio Weber 1
PMCID: PMC433393  PMID: 4515004

Abstract

Flavin 1,N6-ethenoadenine dinucleotide (εFAD) was prepared by the action of chloroacetaldehyde on flavin adenine dinucleotide. This compound, which has two potential fluorophores, ε-adenine and isoalloxazine, shows extremely efficient energy transfer from the former to the latter. The fluorescences of both moieties are greatly diminished in the intact molecule. Determination of the fluorescence yields and lifetimes leads to the conclusion that at 20° in neutral aqueous solution εFAD exists mainly (90%) as an internally complexed or stacked form.

Keywords: FAD; 1,N6-ethenoadenine derivative; fluorescence emission; coenzyme analogue; energy transfer

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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