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. 1973 Apr;70(4):1073–1077. doi: 10.1073/pnas.70.4.1073

Protein Hal: Partial Deletion of a “γ” Immunoglobulin Gene(s) and Apparent Reinitiation at an Internal AUG Codon

Blas Frangione *,*, Loretta Lee †, Edgar Haber †, Kurt J Bloch †
PMCID: PMC433428  PMID: 4577790

Abstract

Protein Hal is a human γ4 heavy-chain disease protein whose molecular weight is reduced from 55,000 to 25,000 in 6 M guanidine due to the lack of disulfide bonds between heavy chains. Studies of aminoacid sequence indicate that it contains a gap of about 240 residues, starting 10 residues from the N-terminal end and including the rest of the Fd fragment, as well as the hinge region. Normal sequence apparently resumes at a methionine residue (position 252) in the second constant domain (CH2) and seems normal from there to the carboxyl end of the molecule. These results imply that reinitiation of translation at an internal AUG codon occurs in protein Hal.

Keywords: heavy-chain disease, aminoacid sequence, internal deletion, methionine 252

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Selected References

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