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. 1973 Apr;70(4):1122–1126. doi: 10.1073/pnas.70.4.1122

Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts

Tereza Imaizumi 1,2, Heidi Diggelmann 1,2, Klaus Scherrer 1,2
PMCID: PMC433439  PMID: 4123930

Abstract

Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a Cot/2 value of 7.5 × 10-4 mol·sec· liter-1; the melting temperature of the hybrid was 86°. Giant nuclear RNA fractions with sedimentation coefficients of more than 50 S formed hybrids of almost equal stability at Cot/2 values of 0.05-0.42 mol·sec· liter-1, indicating amRNA content of 0.3-1.5%. 12S RNA from the same polyribosomes and nuclear giant RNA from HeLa cells did not cross-hybridize. Although a large part of the giant RNA broke down in 99% dimethylsulfoxide gradients, RNA fractions sedimenting faster than 28S rRNA still were found to consist of up to 0.03% globin mRNA sequences. Thus, the mRNA sequences are contained in the covalent structure of giant nuclear precursors, which are termed precursor-mRNA.

Keywords: precursor-mRNA, hemoglobin, RNA-directed DNA polymerase, anti-messenger DNA, hybridization

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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