Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Apr;70(4):1238–1240. doi: 10.1073/pnas.70.4.1238

Fusion of Phospholipid Vesicles with Viable Acholeplasma laidlawii

C W M Grant 1, Harden M McConnell 1,*
PMCID: PMC433466  PMID: 4515622

Abstract

When vesicles of dipalmitoyl phosphatidylcholine produced by sonication are mixed with Acholeplasma laidlawii in neutral buffer, a phenomenon occurs that is most readily explained in terms of fusion of lipid vesicle membrane with the mycoplasma cytoplasmic membrane. The mycoplasma can readily accumulate large quantities of the foreign lipid without loss of viability. The added lipids do not remain in patches but diffuse laterally throughout the mycoplasma membrane.

Keywords: membrane fusion, spin labels, mycoplasma

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hinz H. J., Sturtevant J. M. Calorimetric studies of dilute aqueous suspensions of bilayers formed from synthetic L- -lecithins. J Biol Chem. 1972 Oct 10;247(19):6071–6075. [PubMed] [Google Scholar]
  2. Hubbell W. L., McConnell H. M. Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc. 1971 Jan 27;93(2):314–326. doi: 10.1021/ja00731a005. [DOI] [PubMed] [Google Scholar]
  3. Kornberg R. D., McConnell H. M. Inside-outside transitions of phospholipids in vesicle membranes. Biochemistry. 1971 Mar 30;10(7):1111–1120. doi: 10.1021/bi00783a003. [DOI] [PubMed] [Google Scholar]
  4. Kornberg R. D., McNamee M. G., McConnell H. M. Measurement of transmembrane potentials in phospholipid vesicles. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1508–1513. doi: 10.1073/pnas.69.6.1508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Scandella C. J., Devaux P., McConnell H. M. Rapid lateral diffusion of phospholipids in rabbit sarcoplasmic reticulum. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2056–2060. doi: 10.1073/pnas.69.8.2056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Weiner H. Interaction of a spin-labeled analog of nicotinamide-adenine dinucleotide with alcohol dehydrogenase. I. Synthesis, kinetics, and electron paramagnetic resonance studies. Biochemistry. 1969 Feb;8(2):526–533. doi: 10.1021/bi00830a011. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES