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. 1973 Apr;70(4):1246–1249. doi: 10.1073/pnas.70.4.1246

Direct Measurement of the pK Values of an Alkaline Bohr Group in Human Hemoglobin

J V Kilmartin *, J J Breen †, G C K Roberts ‡, C Ho †
PMCID: PMC433468  PMID: 4515623

Abstract

Proton nuclear magnetic resonance spectra of normal and des-(his 146β) human hemoglobin in the aromatic resonance region have been compared at different pH values. From these measurements, a pK value for histidine 146β of 7.1 in carbonmonoxyhemoglobin and 8.0 in deoxyhemoglobin has been found. These pK values confirm the proposed role of histidine 146β in the alkaline Bohr effect.

Keywords: NMR studies of Hb, histidine titrations

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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