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. 1973 May;70(5):1308–1312. doi: 10.1073/pnas.70.5.1308

Purification of Oncornaviruses by Agglutination with Concanavalin A

Margaret L Stewart *, Donald F Summers *,†, Ruy Soeiro *,†,‡, Bernard N Fields *,†,‡, Jacob V Maizel Jr *
PMCID: PMC433485  PMID: 4514301

Abstract

Concanavalin A (Con A) has been used to rapidly and selectively agglutinate murine and avian oncornavirions from culture medium or plasma. The agglutinated virus was concentrated rapidly and gently by low-speed centrifugation and solubilization with α-methyl mannoside. Infectious virus was purified 2.3 times with respect to nucleic-acid content, and more than 60% of its infectivity was recovered. Infectious particles of densities 1.18 and 1.16 g/cm3 were found in mouse cells infected with Friend virus. Con A reacted only with particles of density 1.16 g/cm3, indicating heterogeneity with respect to carbohydrate content or structure as well as buoyant density. Electron microscopy of virus agglutinated with Con A showed a zone of Con A-glycoprotein complexes averaging 12-15 nm in thickness.

Keywords: murine leukemia virus, phytohemagglutinin, Friend virus

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Selected References

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