Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 May;70(5):1373–1377. doi: 10.1073/pnas.70.5.1373

Synchronous Mating in Yeast

Elissa P Sena 1, David N Radin 1, Seymour Fogel 1
PMCID: PMC433500  PMID: 4576017

Abstract

Homogeneous a and α unbudded yeast cells in logarithmic phase, grown in supplemented minimal medium and isolated by zonal gradient centrifugation, are used for mating. When these cells are resuspended in aerated defined medium, highly synchronous mating rapidly occurs. Within 20 min of incubation at 30° early sexual pairing is evident; extensive agglutination is observed by 60 min, and cell fusion and bud initiation in zygotes occurs after 60-140 min. Sorbitol gradient fractionation of mating mixtures taken at various times during incubation allows the isolation of zygotes or unmated cells. Zygote preparations 90-95% purified are obtained in quantities suitable for genetic and biochemical analysis. The mating procedure is predictable and reproducible.

Keywords: zygote isolation, cell fusion, mating efficiency

Full text

PDF
1373

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crandall M. A., Brock T. D. Molecular basis of mating in the yeast hansenula wingei. Bacteriol Rev. 1968 Sep;32(3):139–163. doi: 10.1128/br.32.3.139-163.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Duntze W., MacKay V., Manney T. R. Saccharomyces cerevisiae: a diffusible sex factor. Science. 1970 Jun 19;168(3938):1472–1473. doi: 10.1126/science.168.3938.1472. [DOI] [PubMed] [Google Scholar]
  3. Foge S., Ortimer R. K. Fidelity of meiotic gene conversion in yeast. Mol Gen Genet. 1970;109(2):177–185. doi: 10.1007/BF00269654. [DOI] [PubMed] [Google Scholar]
  4. Lhoas P. Mating pairs of Saccharomyces cerevisiae infected with double stranded RNA viruses from Aspergillus niger. Nat New Biol. 1972 Mar 22;236(64):86–87. doi: 10.1038/newbio236086a0. [DOI] [PubMed] [Google Scholar]
  5. MORTIMER R. K. Evidence of two types of x-ray-induced lethal damage in Saccharomyces cerevisiae. Radiat Res. 1955 Jun;2(4):361–368. [PubMed] [Google Scholar]
  6. Sakai K., Yanagishima N. Mating reaction in Saccharomyces cerevisiae. II. Hormonal regulation of agglutinability of a type cells. Arch Mikrobiol. 1972;84(3):191–198. doi: 10.1007/BF00425197. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES