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. 1973 May;70(5):1388–1392. doi: 10.1073/pnas.70.5.1388

Effect of Insulin on the Growth Pattern and Adenylate Cyclase Activity of BHK Fibroblasts

Luis Jiménez De Asúa 1,2, Estela S Surian 1,2, Mirtha M Flawia 1,2, Héctor N Torres 1,2,*
PMCID: PMC433503  PMID: 4351176

Abstract

BHK 21/13 fibroblasts grown in the presence of insulin show some characteristics of a transformed strain. Cells broaden, cross each other intricately, and form colonies on both polystyrene dishes and soft agar. The effect of insulin is prevented by addition of N6-butyryl adenosine 3′:5′-cyclic monophosphate and theophylline to the culture medium. Insulin causes a decrease in the concentration of intracellular adenosine 3′:5′-cyclic monophosphate and inhibition of adenylate cyclase activity in BHK fibroblast membranes. This inhibition is observed at insulin concentrations of 0.1 nM or higher.

Keywords: cell transformation, N6-butyryl cyclic AMP, plasma membrane

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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