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. 1973 May;70(5):1397–1400. doi: 10.1073/pnas.70.5.1397

Isolation and Characterization of HL-A Variants in Cultured Human Lymphoid Cells

Donald Pious 1, Pamela Hawley 1, Gary Forrest 1
PMCID: PMC433505  PMID: 4514309

Abstract

We have developed a procedure for immune selection in an established human lymphoid cell line based on HL-A, the major human histocompatibility locus. After a single brief exposure to selective conditions, HL-A2 variant clones were isolated from an HL-A2/HL-A3 heterozygous line. The variant clones occurred at a frequency of about 1 × 10-6. The variant phenotype was stable during prolonged growth in the absence of antiserum after isolation. The variant sublines bound [unk] 1/1000 the HL-A2 antibody per cell as the parent line. Variation was specific in that expression of antigens not selected against was unimpaired. Loss of the chromosome bearing HL-A2 was excluded as the cause of variation because the variants, like the parent line, were heterozygous for phosphoglucomutase (EC 2.7.5.1) determined by structural locus PGM3, which is linked to HL-A.

Keywords: somatic genetics, immune selection, human histocompatibility locus, phosphoglucomutase 3

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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