Abstract
HL-A 2 and HL-A 7 antigens have been solubilized from cultured human lymphocytes by papain and purified on a small radioactive scale after formation of immune complexes, or on a large scale by column chromatographic methods. In both cases, it has been shown that the materials obtained consist of two noncovalently bound fragments. One fragment is a glycopeptide of molecular weight 30,000-31,000, the other is a peptide of molecular weight 11,000-12,000. In polyacrylamide gels containing 8 M urea, the large fragments of HL-A 2 and HL-A 7 exhibited different electrophoretic mobilities, while the small fragments were the same. The relationship of these materials to the native HL-A molecule is discussed.
Keywords: histocompatibility, glycoproteins, membrane, molecular weight
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Colombani J., Colombani M., Viza D. C., Degani-Bernard O., Dausset J., Davies D. A. Separation of HL-A transplantation antigen specificities. Transplantation. 1970 Mar;9(3):228–239. doi: 10.1097/00007890-197003000-00006. [DOI] [PubMed] [Google Scholar]
- Fish W. W., Mann K. G., Tanford C. The estimation of polypeptide chain molecular weights by gel filtration in 6 M guanidine hydrochloride. J Biol Chem. 1969 Sep 25;244(18):4989–4994. [PubMed] [Google Scholar]
- Mann D. L. The effect of enzyme inhibitors on the solubilization of HL-A antigens with 3 M KCl. Transplantation. 1972 Sep;14(3):398–401. [PubMed] [Google Scholar]
- Reisfeld R. A., Pellegrino M. A., Kahan B. D. Salt extraction of soluble HL-A antigens. Science. 1971 Jun 11;172(3988):1134–1136. doi: 10.1126/science.172.3988.1134. [DOI] [PubMed] [Google Scholar]
- SANDERSON A. R. APPLICATIONS OF ISO-IMMUNE CYTOLYSIS USING RADIOLABELLED TARGET CELLS. Nature. 1964 Oct 17;204:250–253. doi: 10.1038/204250a0. [DOI] [PubMed] [Google Scholar]
- Sanderson A. R., Batchelor J. R. Transplantation antigens from human spleens. Nature. 1968 Jul 13;219(5150):184–186. doi: 10.1038/219184a0. [DOI] [PubMed] [Google Scholar]
- Sanderson A. R., Cresswell P., Welsh K. I. Involvement of carbohydrate in the immunochemical determinant area of HL-A substances. Nat New Biol. 1971 Mar 3;230(1):8–12. doi: 10.1038/newbio230008a0. [DOI] [PubMed] [Google Scholar]
- Sanderson A. R. HL-A substances from human spleens. Nature. 1968 Oct 12;220(5163):192–195. doi: 10.1038/220192a0. [DOI] [PubMed] [Google Scholar]
- Schwartz B. D., Nathenson S. G. Isolation of H-2 alloantigens solubilized by the detergent NP-40. J Immunol. 1971 Nov;107(5):1363–1367. [PubMed] [Google Scholar]
- Tanigaki N., Miyakawa Y., Yagi Y., Pressman D. HL-A antigens from hematopoietic cell lines: molecular size and electrophoretic mobility. J Immunol. 1971 Aug;107(2):402–408. [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]