Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 May;70(5):1603–1607. doi: 10.1073/pnas.70.5.1603

Papain-Solubilized HL-A Antigens from Cultured Human Lymphocytes Contain Two Peptide Fragments

Peter Cresswell 1, Mervyn J Turner 1, Jack L Strominger 1
PMCID: PMC433551  PMID: 4514327

Abstract

HL-A 2 and HL-A 7 antigens have been solubilized from cultured human lymphocytes by papain and purified on a small radioactive scale after formation of immune complexes, or on a large scale by column chromatographic methods. In both cases, it has been shown that the materials obtained consist of two noncovalently bound fragments. One fragment is a glycopeptide of molecular weight 30,000-31,000, the other is a peptide of molecular weight 11,000-12,000. In polyacrylamide gels containing 8 M urea, the large fragments of HL-A 2 and HL-A 7 exhibited different electrophoretic mobilities, while the small fragments were the same. The relationship of these materials to the native HL-A molecule is discussed.

Keywords: histocompatibility, glycoproteins, membrane, molecular weight

Full text

PDF
1603

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colombani J., Colombani M., Viza D. C., Degani-Bernard O., Dausset J., Davies D. A. Separation of HL-A transplantation antigen specificities. Transplantation. 1970 Mar;9(3):228–239. doi: 10.1097/00007890-197003000-00006. [DOI] [PubMed] [Google Scholar]
  2. Fish W. W., Mann K. G., Tanford C. The estimation of polypeptide chain molecular weights by gel filtration in 6 M guanidine hydrochloride. J Biol Chem. 1969 Sep 25;244(18):4989–4994. [PubMed] [Google Scholar]
  3. Mann D. L. The effect of enzyme inhibitors on the solubilization of HL-A antigens with 3 M KCl. Transplantation. 1972 Sep;14(3):398–401. [PubMed] [Google Scholar]
  4. Reisfeld R. A., Pellegrino M. A., Kahan B. D. Salt extraction of soluble HL-A antigens. Science. 1971 Jun 11;172(3988):1134–1136. doi: 10.1126/science.172.3988.1134. [DOI] [PubMed] [Google Scholar]
  5. SANDERSON A. R. APPLICATIONS OF ISO-IMMUNE CYTOLYSIS USING RADIOLABELLED TARGET CELLS. Nature. 1964 Oct 17;204:250–253. doi: 10.1038/204250a0. [DOI] [PubMed] [Google Scholar]
  6. Sanderson A. R., Batchelor J. R. Transplantation antigens from human spleens. Nature. 1968 Jul 13;219(5150):184–186. doi: 10.1038/219184a0. [DOI] [PubMed] [Google Scholar]
  7. Sanderson A. R., Cresswell P., Welsh K. I. Involvement of carbohydrate in the immunochemical determinant area of HL-A substances. Nat New Biol. 1971 Mar 3;230(1):8–12. doi: 10.1038/newbio230008a0. [DOI] [PubMed] [Google Scholar]
  8. Sanderson A. R. HL-A substances from human spleens. Nature. 1968 Oct 12;220(5163):192–195. doi: 10.1038/220192a0. [DOI] [PubMed] [Google Scholar]
  9. Schwartz B. D., Nathenson S. G. Isolation of H-2 alloantigens solubilized by the detergent NP-40. J Immunol. 1971 Nov;107(5):1363–1367. [PubMed] [Google Scholar]
  10. Tanigaki N., Miyakawa Y., Yagi Y., Pressman D. HL-A antigens from hematopoietic cell lines: molecular size and electrophoretic mobility. J Immunol. 1971 Aug;107(2):402–408. [PubMed] [Google Scholar]
  11. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES