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. 1973 Jun;70(6):1630–1633. doi: 10.1073/pnas.70.6.1630

Response of Dictyostelium Plasma Membranes to Adenosine 3′:5′-Cyclic Monophosphate

James H Gregg 1, Margaret G Nesom 1
PMCID: PMC433560  PMID: 4352645

Abstract

Myxamoebae of the cellular slime mold Dicytostelium discoideum aggregate in response to a chemotactic substance identified as adenosine 3′:5′-cyclic monophosphate. Upon aggregation cell division is suppressed, the cells become adhesive, and differentiation is initiated. Freeze-fracture studies of myxamoebae were conducted to determine the effect of cyclic AMP and calcium on plasma membrane ultrastructure. The inner surfaces of the plasma membranes exhibited particulate structures whose sizes (43-187 Å) and frequency distribution were determined. Cyclic AMP and calcium induced within 2 hr the formation of particles having average diameters 1.7-times and 1.5-times greater, respectively, than those of the vegetative myxamoebae controls. These data suggest that cyclic AMP mobilizes the intracellular calcium which may be effective in changing plasma membrane structure.

Keywords: cellular slime molds, freeze-fracturing, membrane particle alteration

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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