TABLE IV.

Additional Androgen Validation Studies in Heparinized Human Plasma

Test parameter	ASD	T	DHEA	DHT	AND
Within-sample
% RSD	1.30	1.29	1.28	2.12	2.96
% AR	94.3	98.5	93.7	97.7	105
Dilution QC (25 ng/ml)
% RSD	3.58	2.26	5.24	3.72	6.69
% AR	104	94.4	99.3	97.4	104
Three-cycle freeze–thaw
QC1: % RSD	1.71	0.22	2.71	1.55	2.12
QC1: % Change	−0.70	−3.80	−1.50	−7.30	−6.60
QC3: % RSD	2.92	6.14	5.47	3.34	4.06
QC3: % Change	3.00	−5.30	−9.60	−18.8	−29.6
Preprocessed stability (3.5 hr)
RT: % RSD	3.68	1.82	14.5	6.52	13.1
RT: % Change	−1.70	−3.60	12.0	−8.20	−12.9
37°C: % RSD	3.53	1.52	7.34	3.7	14.3
37°C: % Change	−0.70	−3.00	8.00	−10.7	−19.6
Reinjection stability (23.4 hr)
% RSD	4.88	3.82	4.56	5.73	11.1
% AR	107	97.6	96.3	94.2	108
Extracted sample stability (25.7 hr)
% RSD	5.72	2.60	3.90	3.46	8.90
% AR	107	95.8	94.3	90.8	80.1
Absolute recovery
% RSD	2.00	6.31	5.39	14.3	13.9
% Recovery	106	95.5	114	87.8	89.2

AR, analytical recovery; RSD, relative standard deviation; RT, room temperature.