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. 1973 Jun;70(6):1764–1767. doi: 10.1073/pnas.70.6.1764

A New Form of DNA Polymerase III and a Copolymerase Replicate a Long, Single-Stranded Primer-Template†

William Wickner 1, Randy Schekman 1, Klaus Geider 1, Arthur Korǹberg 1
PMCID: PMC433591  PMID: 4578443

Abstract

A new form of DNA polymerase III, termed Pol III star (Pol III*), has been purified to homogeneity from Escherichia coli. Pol III* is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III. Pol III* and Pol III are separable by gel filtration. Pol III* utilizes a duplex template containing short gaps with the same catalytic properties as Pol III. However, Pol III* is able to replicate long, singlestranded templates such as homopolymer chains and viral circles of M13 and ϕX174 if provided with the following: spermidine, a primer fragment, and a new protein, termed copolymerase III* (Copol III*). The latter, purified to homogeneity, has no known independent enzymatic activity and supports synthesis by Pol III* but not by Pol I, Pol II, or Pol III.

Keywords: DNA replication, M13, ϕX174, spermidine, dnaE mutant

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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