Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Jun;70(6):1891–1893. doi: 10.1073/pnas.70.6.1891

Genetically Related Protein Variants Specifically Associated with Fruiting Body Maturation in Neurospora

June Bowman Nasrallah 1, Adrian M Srb 1
PMCID: PMC433620  PMID: 4269001

Abstract

Electrophoretic analysis of fruiting body extracts from Neurospora reveals a characteristic protein, apparently absent in vegetative structures and ascospores, and which increases markedly in relative concentration after fertilization. Different wild-type species and strains studied have electrophoretic variants of this protein, two of which are shown to be controlled by members of an allelie pair.

Keywords: development, perithecium, electrophoresis

Full text

PDF
1891

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barber J. T., Srb A. M., Steward F. C. Proteins, morphology, and genetics in Neurospora. Dev Biol. 1969 Aug;20(2):105–124. doi: 10.1016/0012-1606(69)90008-6. [DOI] [PubMed] [Google Scholar]
  2. Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Anal Biochem. 1967 Jul;20(1):150–154. doi: 10.1016/0003-2697(67)90272-2. [DOI] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. Gorovsky M. A., Carlson K., Rosenbaum J. L. Simple method for quantitive densitometry of polyacrylamide gels using fast green. Anal Biochem. 1970 Jun;35(2):359–370. doi: 10.1016/0003-2697(70)90196-x. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  7. Urey J. C. Enzyme patterns and protein synthesis during synchronous conidiation in Neurospora crassa. Dev Biol. 1971 Sep;26(1):17–27. doi: 10.1016/0012-1606(71)90103-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES