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. 1973 Jul;70(7):2064–2068. doi: 10.1073/pnas.70.7.2064

Virus-Specific Messenger RNA and Nascent Polypeptides in Polyribosomes of Cells Replicating Murine Sarcoma-Leukemia Viruses

G Vecchio 1,*, N Tsuchida 1, G Shanmugam 1, M Green 1
PMCID: PMC433666  PMID: 4352969

Abstract

We present evidence that virus-specific RNA is present in polyribosomes of transformed cells replicating the murine sarcoma-leukemia virus complex and that it serves as messenger RNA for the synthesis of viral-coded proteins. Both virus-specific RNA (detected by hybridization with the [3H]DNA product of the viral RNA-directed DNA polymerase) and nascent viral polypeptides (measured by precipitation with antiserum to purified virus) were found in membrane-bound and free polyribosomes. Membrane-bound polyribosomes contained a higher content of both virus-specific RNA and nascent viral polypeptides. From 60 to 70% of viral RNA sequences were released from polyribosomes with EDTA, consistent with a function as messenger RNA. Maximum amounts of both virus-specific RNA and nascent viral polypeptides were found in the polyribosome region sedimenting at about 350 S.

Keywords: hybridization, RNA-directed DNA polymerase, membrane-bound polysomes, immunoprecipitation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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