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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Aug;70(8):2189–2192. doi: 10.1073/pnas.70.8.2189

Analysis of Long Pyrimidine Polynucleotides in HeLa Cell Nuclear DNA: Absence of Polydeoxythymidylate

H C Birnboim *, R E J Mitchel *, N A Straus
PMCID: PMC433698  PMID: 4525425

Abstract

HeLa cell nuclear DNA contains an unexpectedly large amount of long pyrimidine polynucleotides. These sequences were detected in DNA labeled with [3H] thymidine by treatment with formic acid-diphenylamine and subsequent analysis by polyacrylamide gel electrophoresis. About 0.5% of the total thymine residues were found in polynucleotides that migrated more slowly than 4S RNA. No polynucleotides of comparable size were detected in Escherichia coli DNA. The pyrimidine polynucleotides contained no pure poly(dT) sequences (less than 0.0015% of the total residues), as judged by thermal chromatography of a complex formed with poly(A).

Keywords: formic acid-diphenylamine hydrolysis, hydroxyapatite chromatography, thermal elution

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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