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. 1973 Aug;70(8):2253–2255. doi: 10.1073/pnas.70.8.2253

Dopamine-β-Hydroxylase: A Tetrameric Glycoprotein

Ellen F Wallace 1, Mark J Krantz *, Walter Lovenberg 1
PMCID: PMC433712  PMID: 4525162

Abstract

Dopamine-β-hydroxylase (EC 1.14.2.1) has been isolated as a pure protein from bovine-adrenal glands. Although the molecular weight of the native protein is well established (290,000), sodium dodecyl sulfate-gel electrophoresis under dissociating conditions gave a single band with a molecular weight of about 75,000. The enzyme contains about 4% carbohydrate, which consists of residues of mannose, glucosamine, galactose, glucose, fucose, and sialic acid. The pure enzyme also contains about 4 atoms of copper per molecule. It is concluded that dopamine-β-hydroxylase is a tetrameric glycoprotein.

Keywords: sodium dodecyl sulfate-gel electrophoresis, molecular weight, transmission of nerve impulses

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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