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. 1973 Aug;70(8):2266–2270. doi: 10.1073/pnas.70.8.2266

Avian Tumor Virus RNA: A Comparison of Three Sarcoma Viruses and Their Transformation-Defective Derivatives by Oligonucleotide Fingerprinting and DNA-RNA Hybridization

Michael M C Lai *,†, Peter H Duesberg *,†, Jürgen Horst *,†,*, Peter K Vogt †
PMCID: PMC433715  PMID: 4365699

Abstract

Earlier electrophoretic analyses have shown that the 60-70S RNA of avian sarcoma viruses contains a characteristic subunit, termed class a subunit, which has a lower electrophoretic mobility than class b subunit found in transformation-defective derivatives of sarcoma viruses and in avian leukosis viruses. We have compared the RNAs of three nondefective avian sarcoma viruses, B77 and Prague and Schmidt-Ruppin strains of Rous sarcoma virus, with those of their transformation-defective (td) derivatives, td B77, td PR-C, and td SR-A, respectively, to determine the chemical basis for the difference between class a and b subunits.

It was found by “fingerprinting” that (1) all (about 20-25) large T1 RNase-resistant oligonucleotides present in class b subunits of transformation-defective viruses have homologous counterparts in the class a subunits of corresponding nondefective sarcoma viruses and that (2) class a subunits contain a few (one or two) additional oligonucleotides that are not present in class b. By contrast the oligonucleotide fingerprints of avian tumor viruses of different strains and subgroups were very different.

Cross hybridization of classes a and b RNA of sarcoma virus B77 with DNA transcribed from a corresponding transformation-defective virus td B77 showed that the two RNAs share at least 60% and differ by about 10% of their sequences.

It is suggested that the structural relationship of class a and b subunits of corresponding viruses may be expressed as a = b + x, and that all the oligonucleotides present only in RNAs of sarcoma viruses but not in transformation-defective viruses of the corresponding strains are part of sequence(s) x. The possibility that x represents genetic information directly or indirectly involved in transformation of fibroblasts is discussed.

Keywords: Rous sarcoma virus, a and b subunits

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brownlee G. G., Sanger F. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969 Dec;11(2):395–399. doi: 10.1111/j.1432-1033.1969.tb00786.x. [DOI] [PubMed] [Google Scholar]
  2. Canaani E., Duesberg P. Role of subunits of 60 to 70S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase. J Virol. 1972 Jul;10(1):23–31. doi: 10.1128/jvi.10.1.23-31.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duesberg P. H., Canaani E. Complementarity between Rous sarcoma virus (RSV) RNA and the in vitro-synthesized DNA of the virus-associated DNA polymerase. Virology. 1970 Nov;42(3):783–788. doi: 10.1016/0042-6822(70)90325-9. [DOI] [PubMed] [Google Scholar]
  4. Duesberg P. H. Physical properties of Rous Sarcoma Virus RNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1511–1518. doi: 10.1073/pnas.60.4.1511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duesberg P. H., Vogt P. K. Differences between the ribonucleic acids of transforming and nontransforming avian tumor viruses. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1673–1680. doi: 10.1073/pnas.67.4.1673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duesberg P. H., Vogt P. K., Martin G. S. The 60-70S RNA of avian sarcoma and leukosis viruses--distribution of class a and b subunits. Bibl Haematol. 1973;39:462–473. doi: 10.1159/000427877. [DOI] [PubMed] [Google Scholar]
  7. Erikson E., Erikson R. L. Association of 4S ribonucleic acid with oncornavirus ribonucleic acids. J Virol. 1971 Aug;8(2):254–256. doi: 10.1128/jvi.8.2.254-256.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Friis R. R., Toyoshima K., Vogt P. K. Conditional lethal mutants of avian sarcoma viruses. I. Physiology of ts 75 and ts 149. Virology. 1971 Feb;43(2):375–389. doi: 10.1016/0042-6822(71)90310-2. [DOI] [PubMed] [Google Scholar]
  9. Horst J., Content J., Mandeles S., Fraenkel-Conrat H., Duesberg P. Distinct oligonucleotide patterns of distinct influenza virus RNA's. J Mol Biol. 1972 Aug 21;69(2):209–215. doi: 10.1016/0022-2836(72)90226-4. [DOI] [PubMed] [Google Scholar]
  10. Horst J., Keith J., Fraenkel-Conrat H. Characteristic two-dimensional patterns of enzymatic digests of oncorna and other viral RNAs. Nat New Biol. 1972 Nov 22;240(99):105–109. doi: 10.1038/newbio240105a0. [DOI] [PubMed] [Google Scholar]
  11. Martin G. S., Duesberg P. H. The a subunit in the RNA of transforming avian tumor viruses. I. Occurrence in different virus strains. II. Spontaneous loss resulting in nontransforming variants. Virology. 1972 Feb;47(2):494–497. doi: 10.1016/0042-6822(72)90287-5. [DOI] [PubMed] [Google Scholar]
  12. NYGAARD A. P., HALL B. D. FORMATION AND PROPERTIES OF RNA-DNA COMPLEXES. J Mol Biol. 1964 Jul;9:125–142. doi: 10.1016/s0022-2836(64)80095-4. [DOI] [PubMed] [Google Scholar]
  13. Robertson H. D., Jeppesen P. G. Extent of variation in three related bacteriophage RNA molecules. J Mol Biol. 1972 Jul 28;68(3):417–428. doi: 10.1016/0022-2836(72)90096-4. [DOI] [PubMed] [Google Scholar]
  14. Toyoshima K., Friis R. R., Vogt P. K. The reproductive and cell-transforming capacities of avian sarcoma virus B77: inactivation with UV light. Virology. 1970 Sep;42(1):163–170. doi: 10.1016/0042-6822(70)90249-7. [DOI] [PubMed] [Google Scholar]
  15. Vogt P. K. Spontaneous segregation of nontransforming viruses from cloned sarcoma viruses. Virology. 1971 Dec;46(3):939–946. doi: 10.1016/0042-6822(71)90092-4. [DOI] [PubMed] [Google Scholar]
  16. Weiss R. A., Friis R. R., Katz E., Vogt P. K. Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology. 1971 Dec;46(3):920–938. doi: 10.1016/0042-6822(71)90091-2. [DOI] [PubMed] [Google Scholar]

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