Abstract
Human parathyroid glands from a patient with chronic renal failure were used to study in vitro biosynthesis of immunoreactive parathyroid hormone. Parathyroid glands were incubated in the presence of [3H]leucine, and classical subcellular fractions were prepared. peptides from acetic acid extractions of the fractions were separated on Sephadex G-75 Superfine. Eluates were assayed for immunoreactive parathyroid hormone by a double-antibody technique. Leucine was incorporated into several large immunoreactive peptides of parathyroid hormone having estimated molecular weights of >25,000, 18,000, and 13,000. The large forms of immunoreactive parathyroid hormone were prominant in the microsomes. The secretion granule fraction, as well as the patient's serum, contained principally immunoreactive parathyroid hormone with an apparent molecular weight of 10,000.
Keywords: protein synthesis, subcellular fractions
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- Cohn D. V., Macgregor R. R., Chu L. L., Kimmel J. R., Hamilton J. W. Calcemic fraction-A: biosynthetic peptide precursor of parathyroid hormone. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1521–1525. doi: 10.1073/pnas.69.6.1521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
- Habener J. F., Kemper B., Potts J. T., Jr, Rich A. Proparathyroid hormone: biosynthesis by human parathyroid adenomas. Science. 1972 Nov 10;178(4061):630–633. doi: 10.1126/science.178.4061.630. [DOI] [PubMed] [Google Scholar]
- Kemper B., Habener J. F., Potts J. T., Jr, Rich A. Proparathyroid hormone: identification of a biosynthetic precursor to parathyroid hormone. Proc Natl Acad Sci U S A. 1972 Mar;69(3):643–647. doi: 10.1073/pnas.69.3.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sorenson R. L., Steffes M. W., Lindall A. W. Subcellular localization of proinsulin to insulin conversion in isolated rat islets. Endocrinology. 1970 Jan;86(1):88–96. doi: 10.1210/endo-86-1-88. [DOI] [PubMed] [Google Scholar]