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. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330

Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat Germ

Bryan E Roberts *, Bruce M Paterson †
PMCID: PMC433729  PMID: 4525168

Abstract

Extracts prepared from commercially available wheat germ efficiently translate messenger RNAs from either viral or eukaryotic origin. The addition of tobacco mosaic virus RNA stimulated amino-acid incorporation more than 100-times and rabbit globin 9S RNA between 20- and 30-times. The in vitro product directed by globin 9S RNA comigrated precisely with authentic rabbit globin on sodium dodecyl sulfate-polyacrylamide gels. Furthermore, during high voltage ionophoresis two [35S]methionine-labeled tryptic peptides synthesized in vitro comigrated in two dimensions with αT5 and βT5 tryptic peptides from authentic [35S]methionine-labeled rabbit globin.

Keywords: wheat germ extracts, protein synthesis, gel electrophoresis, peptide analysis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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