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. 1973 Aug;70(8):2369–2373. doi: 10.1073/pnas.70.8.2369

Studies on In Vitro DNA Synthesis Purification of dna C Gene Product Containing dna D Activity from Escherichia coli*

Sue Wickner 1, Ira Berkower 1, Michel Wright 1, Jerard Hurwitz 1
PMCID: PMC433737  PMID: 4599623

Abstract

The conversion of ϕX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The dna C and dna D gene products copurify, have similar characteristics, are both temperature sensitive in preparations from dna C temperature-sensitive cells, and are both undetectable in preparations from dna D temperature-sensitive cells.

Keywords: ϕX174 DNA, DNA replication, DNA initiation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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