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. 1973 Aug;70(8):2453–2456. doi: 10.1073/pnas.70.8.2453

Detection and Isolation of a New DNA Polymerase from Human Breast Tumor Cell Line HBT-3 by (dT)12-18-Cellulose Chromatography

Brenda I Gerwin 1, Robert H Bassin 1
PMCID: PMC433755  PMID: 4525430

Abstract

Column chromatography on (dT)12-18-cellulose was used to detect and isolate a novel DNA polymerase activity from human breast tumor cell line HBT-3. The new enzyme is similar to those of mammalian RNA tumor viruses in its molecular size, preference for poly(A)·(dT)12-18 as compared to either poly(dA)·(dT)12-18 or poly(A)·poly(dT), and ability to copy poly(C)·(dG)12-18. A similar DNA polymerase activity, defined by its binding to (dT)12-18-cellulose, is not detectable in several other cell lines of human tumors.

Keywords: RNA tumor virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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