Abstract
The growth characteristics of a bromodeoxyuridine-dependent cell line that was derived from a non-contact-inhibited Syrian hamster melanoma line were studied. The dependent cells require high concentrations of bromodeoxyuridine not only for optimal growth but also for the maintenance of the non-contact-inhibited state. When grown in the absence of bromodeoxyuridine, the dependent cells become contact-inhibited. The transition to the contact-inhibited state is reversed when bromodeoxyuridine is added back to the medium, if the bromodeoxyuridine is incorporated into dividing cells. The effects of bromodeoxyuridine on growth rate and on contact inhibition are separable.
Keywords: contact inhibition, melanoma, hamster
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- Bick M. D., Davidson R. L. Total substitution of bromodeoxyuridine for thymidine in the DNA of a bromodeoxyuridine-dependent cell line. Proc Natl Acad Sci U S A. 1974 May;71(5):2082–2086. doi: 10.1073/pnas.71.5.2082. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson R. L., Bick M. D. Bromodeoxyuridine dependence--a new mutation in mammalian cells. Proc Natl Acad Sci U S A. 1973 Jan;70(1):138–142. doi: 10.1073/pnas.70.1.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holley R. W., Kiernan J. A. "Contact inhibition" of cell division in 3T3 cells. Proc Natl Acad Sci U S A. 1968 May;60(1):300–304. doi: 10.1073/pnas.60.1.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Silagi S., Bruce S. A. Suppression of malignancy and differentiation in melanotic melanoma cells. Proc Natl Acad Sci U S A. 1970 May;66(1):72–78. doi: 10.1073/pnas.66.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]