Figure 8. Linear H-NS filaments did not aid in Rho-dependent termination.
(A) Densitometry profiles of transcripts produced at 28°C, 8 mM Mg2+, and 30 µM each NTP from the λPR-bgl template in linear filaments (200 H-NS/kb) with or without 5 nM Rho. Samples were removed at 2, 4, 8, 16, and 32 min after addition of NTPs and separated by denaturing PAGE. Rho-terminated, released transcripts for the 32-min sample were determined as described in the legend to Figure 7. (B) Mean transcript lengths were averaged from two independent experiments.
Figure 8—figure supplement 1. Linear H-NS Filaments did not aid in Rho termination.
(A) Halted A26 ECs (10 nM) formed on λPR-bgl template were incubated with either 66 H-NS/kb or 200 H-NS/kb with or without 5 nM Rho at 8 mM Mg2+ and 28°C. Samples were then collected at 2, 4, 8, 16, and 32 min after addition of NTPs (30 µM each) and resolved by PAGE. Rho-terminated, released transcripts for the 32-min sample were determined as described in the legend to Figure 7. R, released RNAs, M, labeled MspI-digested pBR322 marker. RO, run-off RNAs.