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. 1974 Sep;71(9):3345–3349. doi: 10.1073/pnas.71.9.3345

Purification of Naked Intranuclear Particles from Human Liver Infected by Hepatitis B Virus

Shalom Z Hirschman *, Michael Gerber †,‡, Esther Garfinkel *
PMCID: PMC433768  PMID: 4530305

Abstract

The isolation and purification of the naked intranuclear particles of a human liver infected with hepatitis B virus is described. The particles had a diameter of 27-28 nm, were hexagonal, and showed the presence of capsomeres and spikes with lengths of 7-10 nm projecting from the surfaces. The particles formed cubic arrays with center-to-center distances of 40-42 nm. The overall particle symmetry was icosahedral. The ultraviolet absorption spectrum of the particles showed a peak at 264 nm with a shoulder at 280 nm. Endogenous DNA polymerase activity was not detected in fractions containing particles. DNA polymerase activity was present when activated DNA or d(A-T)n was used as exogenous template.

Keywords: hepatitis B antigen, DNA polymerase, transmission electron microscopy, immunofluorescence

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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