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. 1974 Sep;71(9):3385–3389. doi: 10.1073/pnas.71.9.3385

Cell-Free Translation of Highly Purified Adenovirus Messenger RNA

Larry Eron 1, Heiner Westphal 1
PMCID: PMC433777  PMID: 4530310

Abstract

Several polypeptides contained in the coat and the core of adenovirus type 2 particles have been identified as virus gene products. They are synthesized in vitro by a cell-free protein-making system programmed by adenovirus type 2 messenger RNA that has been hybridized with and eluted from virus DNA. Fractionation by size yields subpopulations of viral messenger RNA that differ in their coding specificities. The procedures outlined in this study may be used to establish a genetic map of adenovirus type 2.

Keywords: polysomal RNA, oligo(dT)-cellulose, RNA·DNA hybridization, Krebs II ascites system

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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