Abstract
Cytological preparations of interphase nuclei and chromosomes from mouse 3T6 cells prepared at various times after infection with the murine sarcomaleukemia virus complex were hybridized with the [3H]DNA product of the viral RNA-directed DNA polymerase. While uninfected nuclei had an average of 4 autoradiographic grains, infected nuclei had 30 grains at 5 hr after infection and 63-65 grains at 11 and 25 hr. Virus-specific grains were localized in the chromocenters of interphase nuclei and were found also in the centromeric heterochromatin region of metaphase chromosomes. These findings provide evidence that the viral RNA-directed DNA polymerase functions to synthesize virus-specific DNA early after infection and that newly synthesized viral DNA rapidly becomes associated with or integrated into specific intranuclear sites.
Keywords: RNA-directed DNA polymerase, transformed cell
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- Aaronson S. A., Todaro G. J., Scolnick E. M. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells. Science. 1971 Oct 8;174(4005):157–159. doi: 10.1126/science.174.4005.157. [DOI] [PubMed] [Google Scholar]
- Arrighi F. E., Hsu T. C., Saunders P., Saunders G. F. Localization of repetitive DNA in the chromosomes of Microtus agrestis by means of in situ hybridization. Chromosoma. 1970;32(2):224–236. doi: 10.1007/BF00286011. [DOI] [PubMed] [Google Scholar]
- Arrighi F. E., Saunders P. P., Saunders G. F., Hsu T. C. Distribution of repetitious DNA in human chromosomes. Experientia. 1971 Aug;27(8):964–966. doi: 10.1007/BF02135776. [DOI] [PubMed] [Google Scholar]
- Baluda M. A., Nayak D. P. DNA complementary to viral RNA in leukemic cells induced by avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1970 Jun;66(2):329–336. doi: 10.1073/pnas.66.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baluda M. A. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576–580. doi: 10.1073/pnas.69.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Britten R. J., Davidson E. H. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(3891):349–357. doi: 10.1126/science.165.3891.349. [DOI] [PubMed] [Google Scholar]
- Eckhardt R. A., Gall J. G. Satellite DNA associated with heterochromatin in Rhynchosciara. Chromosoma. 1971 Mar 16;32(4):407–427. doi: 10.1007/BF00285252. [DOI] [PubMed] [Google Scholar]
- GREEN M. Biochemical studies on adenovirus multiplication. 1. Stimulation of phosphorus incorporation into deoxyribonucleic acid and ribouncleic acid. Virology. 1959 Nov;9:343–358. doi: 10.1016/0042-6822(59)90127-8. [DOI] [PubMed] [Google Scholar]
- Gall J. G., Cohen E. H., Polan M. L. Reptitive DNA sequences in drosophila. Chromosoma. 1971;33(3):319–344. doi: 10.1007/BF00284948. [DOI] [PubMed] [Google Scholar]
- Gall J. G., Pardue M. L. Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A. 1969 Jun;63(2):378–383. doi: 10.1073/pnas.63.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gelb L. D., Aaronson S. A., Martin M. A. Heterogeneity of murine leukemia virus in vitro DNA; detection of viral DNA in mammalian cells. Science. 1971 Jun 25;172(3990):1353–1355. doi: 10.1126/science.172.3990.1353. [DOI] [PubMed] [Google Scholar]
- Green M. Oncogenic viruses. Annu Rev Biochem. 1970;39:701–756. doi: 10.1146/annurev.bi.39.070170.003413. [DOI] [PubMed] [Google Scholar]
- Green M., Rokutanda M., Fujinaga K., Ray R. K., Rokutanda H., Gurgo C. Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses. Proc Natl Acad Sci U S A. 1970 Sep;67(1):385–393. doi: 10.1073/pnas.67.1.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harel L., Harel J., Lacour F., Huppert J. Homologie entre génome du virus de la myéloblastose aviare (AMV) et génome cellulaire. C R Acad Sci Hebd Seances Acad Sci D. 1966 Aug 8;263(6):616–619. [PubMed] [Google Scholar]
- Huebner R. J., Todaro G. J. Oncogenes of RNA tumor viruses as determinants of cancer. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1087–1094. doi: 10.1073/pnas.64.3.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
- John H. A., Birnstiel M. L., Jones K. W. RNA-DNA hybrids at the cytological level. Nature. 1969 Aug 9;223(5206):582–587. doi: 10.1038/223582a0. [DOI] [PubMed] [Google Scholar]
- Jones K. W. Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature. 1970 Mar 7;225(5236):912–915. doi: 10.1038/225912a0. [DOI] [PubMed] [Google Scholar]
- Jones K. W., Robertson F. W. Localisation of reiterated nucleotide sequences in Drosophila and mouse by in situ hybridisation of complementary RNA. Chromosoma. 1970;31(3):331–345. doi: 10.1007/BF00321229. [DOI] [PubMed] [Google Scholar]
- Lowy D. R., Rowe W. P., Teich N., Hartley J. W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 1971 Oct 8;174(4005):155–156. doi: 10.1126/science.174.4005.155. [DOI] [PubMed] [Google Scholar]
- Neiman P. E. Rous sarcoma virus nucleotide sequences in cellular DNA: measurement by RNA-DNA hybridization. Science. 1972 Nov 17;178(4062):750–753. doi: 10.1126/science.178.4062.750. [DOI] [PubMed] [Google Scholar]
- Pardue M. L., Gall J. G. Chromosomal localization of mouse satellite DNA. Science. 1970 Jun 12;168(3937):1356–1358. doi: 10.1126/science.168.3937.1356. [DOI] [PubMed] [Google Scholar]
- Rae P. M. Chromosomal distribution of rapidly reannealing DNA in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1970 Oct;67(2):1018–1025. doi: 10.1073/pnas.67.2.1018. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenthal P. N., Robinson H. L., Robinson W. S., Hanafusa T., Hanafusa H. DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2336–2340. doi: 10.1073/pnas.68.10.2336. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salzberg S., Robin M. S., Green M. Appearance of virus-specific RNA, virus particles, and cell surface changes in cells rapidly transformed by the murine sarcoma virus. Virology. 1973 May;53(1):186–195. doi: 10.1016/0042-6822(73)90477-7. [DOI] [PubMed] [Google Scholar]
- Varmus H. E., Bishop J. M., Vogt P. K. Appearance of virus-specific DNA in mammalian cells following transformation by Rous sarcoma virus. J Mol Biol. 1973 Mar 15;74(4):613–626. doi: 10.1016/0022-2836(73)90052-1. [DOI] [PubMed] [Google Scholar]
- Varmus H. E., Weiss R. A., Friis R. R., Levinson W., Bishop J. M. Detection of avian tumor virus-specific nucleotide sequences in avian cell DNAs (reassociation kinetics-RNA tumor viruses-gas antigen-Rous sarcoma virus, chick cells). Proc Natl Acad Sci U S A. 1972 Jan;69(1):20–24. doi: 10.1073/pnas.69.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss R. A., Friis R. R., Katz E., Vogt P. K. Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology. 1971 Dec;46(3):920–938. doi: 10.1016/0042-6822(71)90091-2. [DOI] [PubMed] [Google Scholar]
- Yoshikawa-Fukada M., Ebert J. D. Hybridization of RNA from Rous sarcoma virus with cellular and viral DNA's. Proc Natl Acad Sci U S A. 1969 Nov;64(3):870–877. doi: 10.1073/pnas.64.3.870. [DOI] [PMC free article] [PubMed] [Google Scholar]