Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Sep;71(9):3445–3449. doi: 10.1073/pnas.71.9.3445

Surface Antigens of Mammalian Sarcoma Virus-Transformed Nonproducer Cells

T Aoki *, J R Stephenson †, S A Aaronson †, K C Hsu ‡
PMCID: PMC433790  PMID: 4139711

Abstract

In vitro, clonal BALB/3T3 mouse cells were transformed into nonproductive sarcoma cells by Kirsten murine sarcoma virus. The resulting cells are called K-BALB. Similarly, the Kirsten sarcoma virus and woolly monkey sarcoma virus transformed normal rat embryonic kidney (NRK) cells to nonproductive sarcoma cells K-NRK and W-NRK, respectively. The presence of sarcoma cell surface antigen (SCSA) on these three different cell lines has been examined by immunoelectron microscopy using the rabbit anti-K-BALB, anti-K-NRK, and anti-W-NRK sera preabsorbed with normal embryonic rat cells, NRK cells, and BALB/3T3 cells. Four different specific sarcoma cell surface antigens have been demonstrated: SCSAa, an antigen specific to the surface of W-NRK cells; SCSAb, a surface antigen common to K-NRK and W-NRK cells; SCSAc, a surface antigen common to K-BALB and W-NRK cells; and SCSAd, a surface antigen broadly reactive with the rabbit antisera to W-NRK, K-NRK, and K-BALB.

Keywords: murine sarcoma virus, woolly monkey sarcoma virus, nonproducer cells, immunoelectron microscopy

Full text

PDF
3445

Images in this article

3447Image
on p.3447

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A. Biologic characterization of mammalian cells transformed by a primate sarcoma virus. Virology. 1973 Apr;52(2):562–567. doi: 10.1016/0042-6822(73)90351-6. [DOI] [PubMed] [Google Scholar]
  2. Aaronson S. A., Rowe S. P. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology. 1970 Sep;42(1):9–19. doi: 10.1016/0042-6822(70)90233-3. [DOI] [PubMed] [Google Scholar]
  3. Aaronson S. A., Todaro G. J. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J Cell Physiol. 1968 Oct;72(2):141–148. doi: 10.1002/jcp.1040720208. [DOI] [PubMed] [Google Scholar]
  4. Aaronson S. A., Weaver C. A. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J Gen Virol. 1971 Nov;13(2):245–252. doi: 10.1099/0022-1317-13-2-245. [DOI] [PubMed] [Google Scholar]
  5. Aoki T., Boyse E. A., Old L. J., De Harven E., Hämmerling U., Wood H. A. G (Gross) and H-2 cell-surface antigens: location on Gross leukemia cells by electron microscopy with visually labeled antibody. Proc Natl Acad Sci U S A. 1970 Mar;65(3):569–576. doi: 10.1073/pnas.65.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Aoki T., Herberman R. B., Johnson P. A., Liu M., Sturm M. M. Wild-type gross leukemia virus: classification of soluble antigens (GSA). J Virol. 1972 Dec;10(6):1208–1219. doi: 10.1128/jvi.10.6.1208-1219.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Aoki T., Hämmerling U., De Harven E., Boyse E. A., Old L. J. Antigenic structure of cell surfaces. An immunoferritin study of the occurrence and topography of H-2' theta, and TL alloantigens on mouse cells. J Exp Med. 1969 Nov 1;130(5):979–1001. doi: 10.1084/jem.130.5.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Aoki T., Stephenson J. R., Aaronson S. A. Demonstration of a cell-surface antigen associated with murine sarcoma virus by immunoelectron microscopy. Proc Natl Acad Sci U S A. 1973 Mar;70(3):742–746. doi: 10.1073/pnas.70.3.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Geering G., Old L. J., Boyse E. A. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J Exp Med. 1966 Oct 1;124(4):753–772. doi: 10.1084/jem.124.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartley J. W., Rowe W. P. Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proc Natl Acad Sci U S A. 1966 Apr;55(4):780–786. doi: 10.1073/pnas.55.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huu Duc-Nguyen, Rosenblum E. N., Zeigel R. F. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J Bacteriol. 1966 Oct;92(4):1133–1140. doi: 10.1128/jb.92.4.1133-1140.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ikeda H., Stockert E., Rowe W. P., Boyse E. A., Lilly F., Sato H., Jacobs S., Old L. J. Relation of chromosome 4 (linkage group 8) to murine leukemia virus-associated antigens of AKR mice. J Exp Med. 1973 Apr 1;137(4):1103–1107. doi: 10.1084/jem.137.4.1103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levy J. P., Varet B., Oppenheim E., Leclerc J. C. Neutralization of Graffi leukaemia virus. Nature. 1969 Nov 8;224(5219):606–608. doi: 10.1038/224606a0. [DOI] [PubMed] [Google Scholar]
  14. Old L. J., Boyse E. A., Stockert E. The G (Gross) leukemia antigen. Cancer Res. 1965 Jul;25(6):813–819. [PubMed] [Google Scholar]
  15. Scolnick E. M., Parks W., Kawakami T., Kohne D., Okabe H., Gilden R., Hatanaka M. Primate and murine type-C viral nucleic acid association kinetics: analysis of model systems and natural tissues. J Virol. 1974 Feb;13(2):363–369. doi: 10.1128/jvi.13.2.363-369.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scolnick E. M., Rands E., Williams D., Parks W. P. Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus. J Virol. 1973 Sep;12(3):458–463. doi: 10.1128/jvi.12.3.458-463.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stephenson J. R., Aaronson S. A. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J Exp Med. 1972 Mar 1;135(3):503–515. doi: 10.1084/jem.135.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stephenson J. R., Aaronson S. A. Murine sarcoma and leukemia viruses: genetic differences determined by RNA-DNA hybridization. Virology. 1971 Nov;46(2):480–484. doi: 10.1016/0042-6822(71)90048-1. [DOI] [PubMed] [Google Scholar]
  19. Stockert E., Old L. J., Boyse E. A. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome. J Exp Med. 1971 Jun 1;133(6):1334–1355. doi: 10.1084/jem.133.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strouk V., Grundner G., Fenyö E. M., Lamon E., Skurzak H., Klein G. Lack of distinctive surface antigen on cells transformed by murine sarcoma virus. J Exp Med. 1972 Aug 1;136(2):344–352. doi: 10.1084/jem.136.2.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Theilen G. H., Gould D., Fowler M., Dungworth D. L. C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. J Natl Cancer Inst. 1971 Oct;47(4):881–889. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES