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. 1974 Sep;71(9):3502–3506. doi: 10.1073/pnas.71.9.3502

Nucleotide Sequence Analysis of RNA Synthesized from Rabbit Globin Complementary DNA

Raymond Poon *, Gary V Paddock *, Howard Heindell *, Philip Whitcome *, Winston Salser *, Dan Kacian †, Arthur Bank ‡, Roberto Gambino ‡, Francesco Ramirez ‡
PMCID: PMC433802  PMID: 4139714

Abstract

Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of 32P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T1 and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the α or β globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants.

Keywords: reverse transcriptase, RNA polymerase, globin mRNA

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Selected References

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